Feline morbillivirus and uses thereof
Described herein are isolated paramyxovirus , a morbillivirus (FmoPV), nucleic acid molecules, polypeptides and antibodies related to FmoPV and uses thereof. In certain embodiments, the FmoPV is a feline morbillivirus . Also described herein is a recombinant FmoPV comprising a modified FmoPV gene or gene segments and uses thereof. Also described is a recombinant FmoPV for the prevention and/or treatment of diseases related to FmoPV or a delivery vector. Also described herein is a diagnostic assay for FmoPV, natural or artificial variants, analogs, or derivatives thereof. Also described herein is a vaccine and a kit containing the vaccine for the prevention and treatment of FmoPV infection. Also provided is a diagnostic kit comprising nucleic acid molecules for the detection of FmoPV.
1. A method of detecting kidney disease in a feline comprising the steps of: (i) obtaining a sample from the feline; (ii) amplifying a DNA molecule derived from the sample using a set of primers that binds to the DNA molecule, wherein the primers are at least 95% identical to the nucleotide sequence of SEQ ID NO: 11, 12, 19, or 20; and (iii) detecting the amplified DNA molecule, which is at least 20 base pair in length, and wherein the feline is infected with a morbillivirus and has kidney disease if the DNA molecule is at least 95% identical to the nucleotide sequence of FmoPV 776U, FmoPV M252A, or FmoPV 761U, and encodes the amino acid sequence of SEQ ID NO:7, 8 or 9, respectively.
2. The method of claim 1 wherein the sample is a cell, blood, serum, plasma, saliva, urine, stool or sputum.
3. The method of claim 1 wherein the kidney disease is tubulointerstitial nephritis (“TIN”).
4. The method of claim 1 wherein the set of primers comprises a primer having the sequence of SEQ ID NO:11 or SEQ ID NO:12.
5. The method of claim 1 wherein the set of primers comprises primers having the sequences of SEQ ID NO:11 and SEQ ID NO:12.
6. The method of claim 1 wherein the set of primers comprises a primer having the sequence of SEQ ID NO:19 or SEQ ID NO:20.
7. The method of claim 1 wherein the set of primers comprises primers having the sequences of SEQ ID NO:19 and SEQ ID NO:20.
8. The method of claim 1 wherein the amplified DNA molecule is less than 200 base pair in length.
9. A method of diagnosing kidney disease in a feline comprising the steps of: (i) obtaining a sample from the feline; (ii) amplifying a DNA molecule derived from the sample using a set of primers that binds to the DNA molecule, wherein the primers are at least 95% identical to the nucleotide sequence of SEQ ID NO: 11, 12, 19, or 20; and (iii) detecting the amplified DNA molecule, which is at least 20 base pair in length, and wherein the feline is infected with a morbillivirus and has kidney disease if the DNA molecule is at least 95% identical to the nucleotide sequence of FmoPV 761U, FmoPV 776U or FmoPV M252A, and encodes the nucleotide sequence of SEQ ID NO: 1, 2 or 3, respectively.