Device and method for detection of analytes

1. A device for detecting the presence or amount of a substance of interest in a liquid sample, the device comprising:

a sample receiving zone for receiving the liquid sample,

wherein the sample receiving zone is present on a porous material;

a sample filtering zone for filtering the liquid sample received at the sample receiving zone,

wherein the sample filtering zone is present on the porous material, and wherein the sample receiving zone and the sample filtering zone are integral with the porous material;

a porous membrane comprising a specific binding pair member at a detection zone that specifically binds to the substance of interest or a substance bound to the substance of interest,

wherein the porous membrane is a separate element from the porous material, and

wherein the porous material and porous membrane are in physical contact over at least an area comprising a portion of the detection zone, and wherein the porous material and porous membrane are in physical contact in a configuration that permits liquid present in the liquid sample to diffuse in, out, through, and about the porous membrane in a substantially random, non-directional manner in an area comprising at least a portion of the detection zone.

2. The device of claim 1 , further comprising a container that contains at least a portion of the porous material and porous membrane.

3. The device of claim 2 , further comprising a porous material comprising a wash solution receiving zone.

4. The device of claim 1 , wherein the specific binding pair member comprises a biological binding pair member.

5. The device of claim 4 , wherein the biological binding pair member comprises a protein, a nucleic acid, biotin, avidin, streptavidin, or combination thereof.

6. The device of claim 4 , wherein the biological binding pair member comprises one or more proteins.

7. The device of claim 6 , wherein the one or more proteins comprises an antibody.