Electrokinetic method for capturing and bioassaying airborne assayable pathogenic agents
Electrokinetic methods are described with the purpose of collecting assayable agents directed by creation of an electrokinetic potential well. Environmental agents such as biowarfare agents, pathogens, allergens or pollutants are collected autonomously. The dielectric fluid medium, such as air, is sampled by electrokinetic propulsion. A further embodiment for collection of pathogen samples entails exposing the sample to an electric plasma in the neighborhood of a high voltage electrode or electrodes, further transported through a potential well created at a sample collection device conductive liquids, such as oils may be sampled for the presence of contaminants, contaminating organisms or bio-degrading organisms.
1. A method for determination of a pathogen or pathogens in an airborne sample volume comprising:
subjecting a wire electrode to a sufficiently high relative voltage to generate an ionizing plasma;
exposing said sample volume to said plasma;
positioning a capture electrode at a low or negative voltage relative to said wire electrode, thereby generating a net air flow, to propel charged particles generated by the ionizing plasma on to the capture electrode;
running said propulsion for a time suitable for air flow of said sample volume; and
performing a biospecific assay to determine presence or amount of said pathogen in said sample volume.
2. The method of claim 1 further comprising a non-conductive transport medium covering said capture electrode to capture the sample volume.
3. The method of claim 2 , wherein said non-conductive transport medium is subject to an extraction procedure with an extraction liquid.
4. The method of claim 3 , wherein said extraction liquid is an NaOH-isopropanol sample treatment reagent.
5. The method of claim 1 , wherein said pathogen is a pathogenic member of the genus bacillus.
6. The method of claim 1 , wherein said pathogen is Mycobacterium tuberculosis.
7. The method of claim 1 , wherein said pathogen is a pathogenic virus.
8. The method of claim 1 , wherein said capture electrode is subject to an extraction procedure with an extraction liquid.
9. The method of claim 8 , wherein said extraction liquid is an NaOH-isopropanol sample treatment reagent.
10. The method of claim 1 , wherein a plurality of capture electrodes at a low or negative voltage are positioned relative to said wire electrode.