Polynucleotide marker genes and their expression, for diagnosis of endotoxemia
The invention discloses isolated endotoxemia marker polynucleotides selected from any one of 163 different polynucleotide sequences, or variants thereof. Endotoxemia related conditions are diagnosed in a test subject by aberrant expression of at least one of the endotoxemia markers or variants thereof. Of practical use, is the early diagnosis of disease, determining those animals at risk of developing endotoxemia, monitoring of an animals immune response to the disease and the enablement of better treatments. Of particular interest is the diagnosis of laminitis in hoofed animals, including horses.
1. A composition comprising a DNA polymerase, whole peripheral blood leukocyte cDNA, wherein the peripheral blood is from a subject with a clinical sign of endotoxemia, and wherein the leukocyte cDNA comprises lysosomal associated membrane protein-1 (LAMP-1) cDNA and matrix metalloproteinase-8 (MMP-8) cDNA; and at least one oligonucleotide primer that comprises a covalently or chemically attached label and that hybridizes to LAMP-1 cDNA.
2. The composition according to claim 1 , wherein the DNA polymerase is a thermostable DNA polymerase.
3. The composition according to claim 2 , wherein the DNA polymerase is Taq polymerase.
4. The composition according to claim 1 , wherein the label comprises a fluorophore.