Functionalized chromatographic materials and methods of making and using therefor
Methods, compositions, devices and kits having a novel chromatographic material are provided herein for separating and identifying organic molecules and compounds, for example molecules and compounds containing electron rich functional groups such as carbon-carbon double bonds. The methods, compositions, and kits include a metal-thiolate chromatographic medium (MTCM) with a sulfur-containing functional group or a metal-selenolate chromatographic medium (MSCM) comprising a selenium-containing functional group covalently attached to a support medium, such that the sulfur-containing functional group or selenium-containing functional group is bound to at least one metal atom. The MTCM and/or MSCM has affinity and specificity to compounds having one or more carbon-carbon double bonds, and performs a highly efficient and rapid separation of samples yielding non-overlapping peaks of purified materials compared to traditional media.
1. A separation system comprising:
a silver thiolate chromatographic medium present as a stationary phase in a pipette or column, wherein the chromatographic medium comprises a sulfur-containing functional group wherein the sulfur is covalently bound to a silver atom, and
wherein the sulfur-containing functional group is linked to a support by at least one spacer selected from: a (C 1 -C 15 )alkyl, a (C 1 -C 15 )alkoxy, a (C 1 -C 15 )heteroalkyl, a (C 6 -C 10 )aryl, a (C 1 -C 9 )heteroaryl, and a (C 6 -C 10 )aryl(C 1 -C 6 )alkyl, and
a sample source coupled to the pipette or column comprising a sample to be separated, wherein the sample includes a compound having a functional group selected from the group consisting of alkenyls, alkynyls, aromatics, and amines.
2. The chromatographic medium according to claim 1 , on a support selected from the group consisting of: silica gel, alumina, polystyrene, agarose, modified polymeric resin, cellulose, magnesium silicate, dextran, and starch.
3. The chromatographic medium according to claim 1 , configured as an analytical component of a chromatographic separation system selected from: normal phase chromatography, reversed-phase chromatography, liquid chromatography, planar chromatography, column chromatography, flush chromatography, flash chromatography, thin layer chromatography, high performance liquid chromatography, gas chromatography, and solid phase extraction chromatography.