Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polypeptide, which has at least 98% sequence identity to the sequence of amino acids 21 to 455 of SEQ ID NO: 2 or is a fragment of the sequence of amino acids 21 to 455 of SEQ ID NO: 2, wherein the polypeptide has cellobiohydrolase activity.
2. The polypeptide of claim 1 , which is a variant of the sequence of amino acids 21 to 455 of SEQ ID NO: 2, comprising a substitution, deletion, and/or insertion of one or more amino acids.
3. The polypeptide of claim 1 , comprising or consisting of the sequence of SEQ ID NO: 2.
4. The polypeptide of claim 1 , comprising or consisting of the sequence of amino acids 21 to 455 of SEQ ID NO: 2.
5. The polypeptide of claim 1 , which is encoded by a polynucleotide that hybridizes with the mature polypeptide coding sequence of SEQ ID NO: 1 or the cDNA sequence thereof under high stringency conditions.
6. The polypeptide of claim 1 , which is a fragment of the sequence of amino acids 21 to 455 of SEQ ID NO: 2.
7. The polypeptide of claim 1 , further comprising a cellulose binding domain.
8. A composition comprising the polypeptide of claim 1 and one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an expansin, an esterase, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
9. A method for degrading or converting a cellulosic material, comprising: treating the cellulosic material with a polypeptide of claim 1 , an endoglucanase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
10. The method of claim 9 , wherein the cellulosic material is pretreated.
11. The method of claim 9 , further comprising recovering the degraded cellulosic material.
12. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with a polypeptide of claim 1 , an endoglucanase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.
13. The method of claim 12 , wherein the cellulosic material is pretreated.
14. The method of claim 12 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
15. The method of claim 12 , wherein the fermentation product is an alcohol, an organic acid, a ketone, an amino acid, or a gas.
16. An isolated polypeptide comprising a catalytic domain, which has at least 99% sequence identity to the sequence of amino acids 102 to 455 of SEQ ID NO: 2 or is a fragment of the sequence of amino acids 102 to 455 of SEQ ID NO: 2, wherein the catalytic domain has cellobiohydrolase activity.
17. The polypeptide of claim 16 wherein the catalytic domain has the sequence of amino acids 102 to 455 of SEQ ID NO: 2.
18. The polypeptide of claim 16 , further comprising a cellulose binding domain.
19. A composition comprising a polypeptide of claim 16 and one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an expansin, an esterase, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.
20. A method for degrading or converting a cellulosic material, comprising: treating the cellulosic material with a polypeptide of claim 16 , an endoglucanase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity.
21. The method of claim 20 , wherein the cellulosic material is pretreated.
22. The method of claim 20 , further comprising recovering the degraded cellulosic material.
23. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with a polypeptide of claim 16 , an endoglucanase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.
24. The method of claim 23 , wherein the cellulosic material is pretreated.
25. The method of claim 23 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
26. The method of claim 23 , wherein the fermentation product is an alcohol, an organic acid, a ketone, an amino acid, or a gas.