Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide in an expression host, and wherein the polypeptide:
(a) has at least 95% sequence identity to the sequence of amino acids 19-465 of SEQ ID NO: 2, or
(b) is a fragment of the sequence of amino acids 19-465 of SEQ ID NO: 2,
wherein the polypeptide has cellobiohydrolase activity.
2. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to the sequence of amino acids 19-465 of SEQ ID NO: 2.
3. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity is a variant of the sequence of amino acids 19-465 of SEQ ID NO: 2, comprising a substitution, deletion, and/or insertion of one or more amino acids, wherein the variant has at least 98% sequence identity to the sequence of amino acids 19-465 of SEQ ID NO: 2.
4. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises the sequence of amino acids 19-465 of SEQ ID NO: 2.
5. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity is a fragment of the sequence of amino acids 19-465 of SEQ ID NO: 2.
6. The nucleic acid construct of claim 2 , wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.
7. The nucleic acid construct of claim 3 , wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.
8. The nucleic acid construct of claim 4 , wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.
9. The nucleic acid construct of claim 5 , wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.
10. A process for degrading a cellulosic material, comprising treating the cellulosic material with a polypeptide having cellobiohydrolase activity, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity,
wherein the polypeptide having cellobiohydrolase activity:
(a) has at least 95% sequence identity to the sequence of amino acids 19-465 of SEQ ID NO: 2, or
(b) is a fragment of the sequence of amino acids 19-465 of SEQ ID NO: 2.
11. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with an enzyme composition in the presence of a polypeptide having cellobiohydrolase activity, an endoglucanase, a beta-glucosidase, and a GH61 polypeptide having cellulolytic enhancing activity; and
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product,
wherein the polypeptide having cellobiohydrolase activity:
(i) has at least 95% sequence identity to the sequence of amino acids 19-465 of SEQ ID NO: 2, or
(ii) is a fragment of the sequence of amino acids 19-465 of SEQ ID NO: 2.
12. The process of claim 11 , further comprising recovering the fermentation product.
13. The process of claim 11 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to the sequence of amino acids 19-465 of SEQ ID NO: 2.
14. The process of claim 13 , wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.
15. The process of claim 14 , further comprising recovering the fermentation product.
16. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 95% sequence identity to the sequence of amino acids 19-465 of SEQ ID NO: 2.
17. The nucleic acid construct of claim 16 wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.
18. A recombinant host cell comprising the nucleic acid construct of claim 1 .
19. The process of claim 10 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to the sequence of amino acids 19-465 of SEQ ID NO: 2.
20. The process of claim 19 , wherein the polypeptide having cellobiohydrolase activity further comprises a cellulose binding domain.