Modulation of receptor-like kinases for promotion of plant growth
View Patent ↗The invention provides the disclosure of a novel plant growth pathway involving several receptor like kinases. The pathway was shown to work largely independent of brassinosteroids and involves several members of the CrRLK1L family of receptor kinases. According to the invention, HERK1, HERK2, FER, THE1 and the brassinosteroid BES1 may be modulated to influence plant growth and elongation. The invention includes methods, and transformed plants, cells tissues and seeds with increased cellular elongation and other related yield traits.
1. A genetically modified plant having increased plant growth and cellular elongation comprising:
a heterologous polynucleotide sequence which encodes a CrRLK1L receptor like kinase polypeptide operably linked to a promoter sequence, wherein said CrRLK1L receptor like kinase is HERCULES 1 Receptor Kinase 1 (HERK1),
wherein said HERK1 comprises one or more of the following sequences:
(a) a nucleic acid comprising the polynucleotide sequence of SEQ ID NO: 5, or a complete complement thereof;
(b) a nucleic acid comprising at least 99% sequence identity to SEQ ID NO: 5, or a complete complement thereof;
(c) a nucleic acid encoding a polypeptide sequence at least 95% identical to SEQ ID NO: 6, wherein said sequences are operably linked to a promoter,
wherein said CrRLK1L receptor like kinase activity is increased, and wherein said modified plant has increased plant growth and cellular elongation when compared to a nonmodified plant.
2. Seed of the plant of claim 1 wherein said seed comprises the heterologous polynucleotide sequence which encodes a CrRLK1L receptor like kinase polypeptide operably linked to a promoter sequence, wherein said CrRLK1L receptor like kinase is HERK1.
3. A method for increasing plant growth and cellular elongation comprising:
increasing the activity of a CrRLK1L receptor like kinase in a plant, wherein said CrRLK1L receptor like kinase HERCULES 1 Receptor Kinase 1 (HERK1);
introducing into the plant an expression cassette comprising a HERK1 nucleic acid operably linked to a promoter that functions in plants, wherein said CrRLK1L receptor like kinase activity is increased;
wherein said HERK1 comprises one or more of the following sequences:
(a) a nucleic acid comprising the polynucleotide sequence of SEQ ID NO: 5, or a complete complement thereof;
(b) a nucleic acid encoding a polypeptide sequence at least 95% identical to SEQ ID NO: 6, wherein said sequences are operably linked to a promoter; and
selecting for plants that have increased plant growth or cellular elongation.
4. The method of claim 3 , wherein said CrRLK1L receptor like kinase activity is increased by increasing the expression of said HERK1 nucleic acid.
5. The method of claim 3 , wherein the expression cassette is introduced by a method selected from the group consisting of: electroporation, micro-projectile bombardment and Agrobacterium -mediated transfer.
6. The method of claim 3 wherein the promoter that functions in plants is a tissue-preferred promoter, tissue-specific promoter or an inducible promoter.
7. The method of claim 3 wherein the plant is Arabidopsis , wheat, rice, sorghum, barley, oat, lawn grass, rye, soybean, canola, Brassica , sunflower, maize, sorghum, alfalfa, cotton, millet, peanut or cacao.
8. A method for increasing plant growth comprising, introducing to said plant an expression construct comprising a CrRLK1L receptor like kinase of one or more of the following sequences:
(a) a nucleic acid comprising the polynucleotide sequence of SEQ ID NO: 5 or a complete complement thereof;
(b) a nucleic acid comprising at least 99% sequence identity to SEQ ID NO: 5 or a complete complement thereof;
(c) a nucleic acid encoding the polypeptide sequence of SEQ ID NO: 6; wherein said sequences are operably linked to a promoter; wherein said CrRLK1L receptor like kinase activity is increased; and selecting for plants that have increased plant growth.
9. The method of claim 8 wherein said promoter is a tissue specific promoter.