Methods for measuring degree of cure or solidification of a composition

1. A method of measuring the degree of polymerization of a polymerizable adhesive composition comprising:

(i) mixing a wavelength-shift fluorophore comprising 7-diethylamino-4-methyl -coumarin with the polymerizable adhesive composition;

(ii) applying the polymerizable adhesive composition to substrates to be bonded to form a bondable assembly;

(iii) permitting polymerization of the polymerizable adhesive composition to bond the substrates to be bonded to form a bonded assembly;

(iv) measuring fluorescence intensity at two emission wavelengths, one on each side of peak fluorescence intensity of the wavelength-shift fluorophore, and determining a ratio therefrom; and

(v) comparing the ratio to predetermined reference values that correlate the ratio of peak fluorescence intensity to (a) a known degree of polymerization of the polymerizable adhesive composition obtained through measurements of the degree of polymerization of the polymerizable adhesive composition and/or (b) a known degree of performance properties of the polymerizable adhesive composition obtained through measurements of the performance properties of the polymerizable adhesive composition.

2. The method of claim 1 , wherein the two emission wavelengths are near or at the minimum and maximum emission wavelengths of the wavelength-shift fluorophore.

3. The method of claim 1 , wherein step (iv) and step (iv) are performed more than once as the polymerizable adhesive composition undergoes polymerization.

4. The method of claim 1 , wherein the polymerizable composition comprises a (meth)acrylate, a urethane, a polyester, a silicone, a polyolefin, an epoxy, or a combination of two or more thereof in either a mixture or copolymer.

5. The method of claim 1 , wherein one or more reference value(s) is obtained by Fourier transform infrared spectroscopy double bond conversion.

6. The method of claim 1 , wherein one or more reference value(s) is a strength property or biocompatibility characteristic.

7. The method of claim 1 , wherein one or more reference value(s) is in the form of a graph.

8. The method of claim 1 , wherein the reference value is a control standard for the wavelength-shift fluorophore.

9. A method of measuring the degree of solidification of a thermoplastic adhesive composition comprising:

(i) mixing a wavelength-shift fluorophore comprising 7-diethylamino-4-methyl -coumarin with the thermoplastic adhesive composition;

(ii) permitting solidification of the thermoplastic adhesive composition;

(ii) measuring fluorescence intensity at two emission wavelengths, one on each side of peak fluorescence intensity, of the wavelength-shift fluorophore and determining a ratio therefrom; and

(iv) comparing the ratio to one or more reference value(s) that correlates the ratio to a known degree of solidification of the thermoplastic adhesive composition.

10. A system for non-destructively determining the degree of polymerization of a polymerizable adhesive composition or solidification of a thermoplastic adhesive composition comprising:

(i) a detection apparatus configured to detect fluorescence intensity of a wavelength-shift fluorophore present in the polymerizable adhesive composition or thermoplastic adhesive composition wherein the wavelength-shift fluorophore is exited by an excitation source and wherein the fluorophore is a fluorophore comprising 7-diethylamino-4-methyl-coumarin;

(ii) an analyzer configured to determine a ratio of fluorescence intensity based on fluorescence intensity at two emission wavelengths, one on each side of peak fluorescence intensity, of the wavelength-shift fluorophore; and

(iii) a correlating device configured to correlate the ratio with the degree of polymerization of the polymerizable composition or solidification of the thermoplastic adhesive composition.

11. The system of claim 10 , further comprising an excitation source configured to emit one or more light wavelengths capable of exciting the wavelength-shift fluorophore.

12. The system of claim 10 , wherein the detection apparatus measures fluorescence intensity at two emission wavelengths, one on each side of peak fluorescence intensity, of the wavelength-shift fluorophore.

13. The system of claim 10 , further comprising a communication interface configured to communicate the degree of polymerization to an external device.

14. The system of claim 10 , further comprising one or more reference value(s) that correlate the ratio with the degree of polymerization of the polymerizable composition or solidification of the thermoplastic adhesive composition.

15. The method of claim 1 , wherein the substrates are each constructed from polycarbonate.

16. The method of claim 1 , wherein one of the substrates is constructed from polycarbonate and the other substrate is constructed from stainless steel.

17. The method of claim 1 , wherein one of the substrates is a cannula and the other substrate is a needle.

18. The method of claim 1 , wherein step (iii) occurs after exposure of the bondable assembly to actinic radiation.