A composition is provided that includes Bacillus subtilis 2084 (NRRL B-50013) or a strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134). Animal bedding that includes the Bacillus strains is also provided, as well as a method of making the animal bedding. Also provided are methods of controlling odors from animal waste. A method of making a composition including the Bacillus strains is also provided.
1. A method comprising:
(a) growing each of Bacillus subtilis 2084 (NRRL B-50013) or a strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134) in a broth; and
(b) separating each of the strains from its broth, wherein each of the separated strains are combined together to form a composition.
2. The method of claim 1 , further comprising freeze drying each strain after separating each strain from its broth.
3. The method of claim 2 , further comprising adding the freeze-dried strain to at least one water-soluble carrier.
4. The method of claim 2 , further comprising adding the freeze-dried strain to at least one carrier that is capable of binding ammonia.
5. The method of claim 1 comprising growing each of Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134) in a broth.
6. The method of claim 1 comprising growing each of Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134) in a broth.
7. The method of claim 1 comprising growing each of Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134).
8. The method of claim 3 , further comprising using a water-soluble carrier selected from the group consisting of: Baker's sugar, maltodextrin M100, and baylith.
9. The method of claim 4 , further comprising using a carrier selected from the group consisting of: limestone, clay, zeolite, diatomaceous earth, clintpolite, bentonite, and Fuller's earth.
10. The method of claim 1 , further comprising adding an absorbent material to the separated strains.
11. The method of claim 10 , wherein adding an absorbent material comprises adding cat litter.
12. A method of making a composition, the method comprising:
(a) growing each of Bacillus subtilis 2084 (NRRL B-50013) or a strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134) in a broth; and
(b) separating each of the strains from its broth;
(c) freeze-drying each separated strain; and
(d) mixing about equal amounts of each freeze-dried strain to make a composition.