Nucleotide sequences encoding RAMOSA3 and sister of RAMOSA3 and methods of use for same
The invention relates to the isolation and characterization of a maize gene, RAMOSA3 (RA3), responsible for meristem development and inflorescence development including branching. The gene, gene product, and regulatory regions may be used to manipulate branching, meristem growth, inflorescence development and arrangement, and ultimately to improve yield of plants. The invention includes the gene and protein product as well as the use of the same for temporal and spatial expression in transgenic plants to alter plant morphology and affect yield in plants. The invention also includes the gene and protein product for SISTER OF RAMOSA3 (SRA).
1. A recombinant DNA construct comprising a polynucleotide operably linked to a heterologous promoter that is functional in a plant, wherein the polynucleotide comprises:
(a) a nucleic acid sequence encoding a polypeptide having trehalose-6-phosphate phosphatase activity, wherein the polypeptide has an amino acid sequence of at least 90% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:19, 49, 68 or 69; or
(b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
2. A method for altering trehalose-6-phosphate phosphatase activity in a plant, comprising:
(a) introducing into a regenerable plant cell the recombinant DNA construct of claim 1 to produce a transformed plant cell; and
(b) regenerating a transgenic plant from said transformed plant cell, wherein said transgenic plant comprises in its genome said recombinant DNA construct and wherein said transgenic plant exhibits an alteration in trehalose-6-phosphate phosphatase activity, when compared to a control plant not comprising said recombinant DNA construct.
3. The method of claim 2 , further comprising (c) obtaining a progeny plant derived from said transgenic plant, wherein said progeny plant comprises in its genome the recombinant DNA construct.
4. The method of claim 2 , wherein the transgenic plant exhibits an increase in trehalose-6-phosphate phosphatase activity.
5. A method for increasing environmental stress tolerance of a plant, comprising:
(a) introducing into a regenerable plant cell the recombinant DNA construct of claim 1 to produce a transformed plant cell; and
(b) regenerating a transgenic plant from said transformed plant cell, wherein said transgenic plant comprises in its genome said recombinant DNA construct and wherein said transgenic plant exhibits an increase in environmental stress tolerance, when compared to a control plant not comprising said recombinant DNA construct.
6. The method of claim 5 , further comprising (c) obtaining a progeny plant derived from said transgenic plant, wherein said progeny plant comprises in its genome the recombinant DNA construct.
7. The method of claim 5 , wherein said environmental stress is drought, and wherein said transgenic plant exhibits an increase in drought tolerance.
8. The recombinant DNA of claim 1 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:19, 49, 68 or 69.
9. The recombinant DNA of claim 1 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:19, 49, 68 or 69.
10. The recombinant DNA of claim 1 , wherein the nucleotide sequence comprises SEQ ID NO:18, 48 or 67.
11. A plant or seed comprising the recombinant DNA construct of claim 1 .
12. A plant or seed comprising the recombinant DNA construct of claim 8 .
13. A plant or seed comprising the recombinant DNA construct of claim 9 .
14. A plant or seed comprising the recombinant DNA construct of claim 10 .
15. The plant or seed of claim 11 , wherein the plant or seed is a maize plant or seed.
16. The plant or seed of claim 12 , wherein the plant or seed is a maize plant or seed.
17. The plant or seed of claim 13 , wherein the plant or seed is a maize plant or seed.
18. The plant or seed of claim 14 , wherein the plant or seed is a maize plant or seed.
19. The method of claim 4 , wherein the plant is maize.
20. The method of claim 7 , wherein the plant is maize.