Programmable microfluidic systems and related methods
A microfluidic device is programmable so that a single microarchitecture design can run many assays. Specifically, the programmable microfluidic device includes an execution method to facilitate translating from a programming language to a set of requests that are specified for the device. In addition, the microfluidic device includes a contamination mitigation method that includes a conflict list to mitigate contamination effects within the microfluidic device.
1. A method to facilitate translating from a programming language to a set of execution requests for a programmable microfluidic chip, comprising the steps of:
reading a description file that includes information regarding hardware components of the programmable microfluidic chip including a number of reservoirs, a number of fluidic functional units, and a number of channels connected to a set of external hardware instrumentation;
receiving a program file comprising a sequence of instructions;
generating a sequence of execution requests by translation based on the description file and the program file;
interfacing between the set of execution requests and the hardware components of the programmable microfluidic chip by mapping each execution request of the sequence to a single state of hardware components;
executing the sequence of execution requests;
triggering the hardware components of the programmable microfluidic chip according to said executing step; and
activating the external hardware instrumentation.
2. The method according to claim 1 wherein the sequence of execution requests includes one or more of: setting up a fluidic path, actuating a pump, effecting a fluidic functional unit, and receiving data from a fluidic functional unit.
3. The method according to claim 1 wherein each execution request corresponds to one or more bit strings.
4. The method of claim 3 , wherein at least one bit string designates a closed or open valve.
5. The method according to claim 1 wherein each execution request includes a pump value corresponding to a pump.
6. The method according to claim 5 wherein the pump value is selected from the group consisting of a closed pump, an open pump, an open pump with a forward pumping action, and an open pump with a reverse pumping action.
7. The method according to claim 1 wherein each execution request includes a number of actuations of a pump.
8. The method according to claim 1 wherein each execution request includes a sensor control.
9. The method according to claim 8 wherein the sensor control is selected from the group consisting of ignore, wait until a fluid is at sensor location, and wait until fluid leaves sensor location.
10. The method according to claim 8 wherein each execution request includes a threshold value.
11. The method according to claim 10 further comprising a sensor value wherein the sensor value exceeds the threshold value or fails to meet the threshold value.
12. The method according to claim 1 wherein the external hardware instrumentation includes one or more sensors, cameras, microscopes, microscope stages, or a temperature control element.
13. The method according to claim 1 wherein each execution request includes one or more of a pump stroke sequence and a pump frequency.
14. The method according to claim 1 wherein a fluidic functional unit is one selected from the group consisting of a mixer, a heater, a separator, a sensor, a detection unit, an actuator, a valve, a pump, an incubation chamber, a reaction chamber, a separation unit, and a control unit.
15. The method of claim 1 , wherein the mapping of each execution request of the sequence to a single state of hardware components includes a termination condition to maintain the single state.
16. The method of claim 15 , wherein the termination condition may include a duration of time, an electrical signal, a sensor value, or an external hardware instrumentation trigger.
17. The method of claim 1 wherein the generated sequence of execution requests is generated using a computer, server, dedicated hardware or embedded system.
18. The method of claim 1 , wherein the execution requests control the fluidic functional units using pneumatics, electrical signals, or external mechanical or electrical devices by transmitting controls signals.
19. The method of claim 18 , wherein the control signals are transmitted to the fluidic functional units or external instrumentation using tubes, wires, computer networks, serial cable, parallel cable, or wirelessly.
20. The method of claim 1 , wherein the hardware description file includes an enumeration of fluidic functional units, the number of each of the fluidic functional units, a description of each of the fluidic functional units, and the connectivity between the fluidic functional units.