Highly sensitive system and method for analysis of troponin
The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.
1. A method for determining a characteristic of cardiovascular health, comprising:
(a) determining a threshold concentration of at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) in blood, serum or plasma in a healthy population, wherein the threshold concentration is less than about 10 pg/ml;
(b) measuring the concentration of the at least one of cTnI and cTnT in a blood, serum or plasma sample from the subject in a single molecule counting assay having a limit of detection for the at least one of cTnI and cTnT of less than 3 pg/mL and a coefficient of variation of less than 10%, wherein the assay comprises contacting the sample with an antibody specific for the at least one of cTnI and cTnT and determining the amount of specific binding between the antibody and the at least one of cTnI and cTnT,
(c) determining a characteristic of cardiovascular health in the individual when the concentration of the at least one of cTnI and cTnT in the sample is greater than concentration of the at least one of cTnI and cTnT in the threshold concentration.
2. The method of claim 1 , wherein the characteristic of cardiovascular health is at least one of acute myocardial infarction, risk of myocardial infarction, cardiac damage, progression of cardiac damage, cardiac toxicity, likelihood of a cardiac event, acute coronary syndrome, myocarditis, heart failure, and likelihood of heart failure.
3. The method of claim 1 , wherein the threshold concentration is within the range of 1 pg/ml to 10 pg/ml.
4. The method of claim 1 , wherein the threshold concentration is less than about 7 pg/ml.
5. The method of claim 1 , wherein the threshold concentration is less than about 8 pg/ml.
6. The method of claim 1 , wherein the threshold concentration is less than about 9 pg/ml.
7. The method of claim 1 , wherein the sample is taken during a stress test.
8. The method of claim 1 , wherein the sample is taken after a stress test.
9. The method of claim 1 , wherein the assay detects a photon emission from a label corresponding to a single molecule of the at least one of cTnI and cTnT in the sample, wherein the at least one antibody is bound to or was bound to a molecule of the at least one of cTnI and cTnT in the sample.
10. The method of claim 9 , wherein the antibody is eluted from the at least one of cTnI or cTnT before the photon emission is detected.
11. The method of claim 9 , wherein the photon emission is detected in a single molecule analyzer.
12. The method of claim 1 , wherein the assay is conducted using an analyzer comprising:
(a) an electromagnetic radiation source for stimulating a fluorescent moiety; and
(b) an electromagnetic radiation detector that measures an electromagnetic characteristic of the fluorescent moiety when stimulated by the electromagnetic radiation.
13. The method of claim 1 wherein step (b) further comprises assessing a non-troponin indicator in the sample, and step (c) comprises determining a characteristic of cardiovascular health based on the concentration of the at least of one the cTnI and cTnT and the assessment of the non-troponin indicator in said sample.
14. The method of claim 13 , wherein the non-troponin indicator is at least one marker selected from the group consisting of creatine kinase (CK), myocardial fraction CK myocardial band (MB), aspartate aminotransferase, lactate dehydrogenase (LDH), α-hydroxybutyrate dehaydrogenase, myoglobin, glutamate oxaloacetate transaminase, glycogen phosphorylase BB, unbound free fatty acids, heart fatty acid binding protein (H-FABP), ischemia-modified albumin, myosin light chain 1, myosin light chain 2.
15. The method of claim 13 , wherein the non-troponin indicator is at least one marker selected from the group consisting of C-reactive protein (CRP), white blood cell count, soluble CD40 ligand, myeloperoxidase, monocyte chernoattractant protein-1, whole blood choline, and pregnancy-associated plasma protein A.
16. The method of claim 13 , wherein the non-troponin indicator is at least one marker selected from the group consisting of Interieukin 8, Interleukin 1β, Interleukin 6, Interleukin 10, Tumor Necrosis Factor, and Interleukin 12p70.
17. A method for determining a characteristic of cardiovascular health, comprising:
(a) measuring the concentration of at least one of cTnI and cTnT in a series of samples blood, serum or plasma samples from the subject in a single molecule counting assay having a limit of detection for the at least one of cTnI and cTnT of less than 3 pg/mL and a coefficient of variation of less than 10%, wherein the assay comprises contacting the samples with an antibody specific for the at least one of cTnI and cTnT and determining the amount of specific binding between the antibody and the at least one of cTnI and cTnT,
(b) determining a characteristic of cardiovascular health in the individual when the concentration of the at least one of cTnI and cTnT is increasing above a threshold concentration in the series samples, and wherein the threshold concentration is less than 10 pg/mL.
18. The method of claim 17 , wherein the characteristic of cardiovascular health is at least one of acute myocardial infarction, risk of myocardial infarction, cardiac damage, progression of cardiac damage, cardiac toxicity, likelihood of a cardiac event, acute coronary syndrome, myocarditis, heart failure and likelihood of heart failure.
19. The method of claim 17 , wherein the method is performed periodically over a period of up to about 30 hours on samples from the same patient taken at different times during the 30 hour period.
20. The method of claim 17 , wherein an interval between samples is less than about 4 hours.
21. The method of claim 17 wherein a rate of change in the level of the at least one of the cTnI and cTnT between individual samples in the series of samples is detected.
22. The method of claim 17 , wherein the threshold concentration represents the 99 th percentile concentration of the at least one of cTnI and cTnT in a population of healthy individuals.
23. The method of claim 17 , wherein the threshold concentration of cTnI is within the range of 1 pg/ml to 10 pg/ml.
24. The method of claim 17 , wherein the threshold concentration is less than about 7 pg/ml.
25. The method of claim 17 , wherein the threshold concentration is less than about 8 pg/ml.
26. The method of claim 17 , wherein the threshold concentration is less than about 9 pg/ml.
27. The method of claim 17 , wherein the assay detects a photon emission from a label corresponding to a single molecule of the at least one of cTnI and cTnT in the sample, wherein the antibody is bound to or was bound to a molecule of the at least one of cTnI and cTnT in the sample.
28. The method of claim 27 , wherein the antibody is eluted from the at least one of cTnI or cTnT before the photon event.
29. The method of claim 27 , wherein the photon emission is detected in a single molecule analyzer.
30. The method of claim 17 , wherein the assay is conducted using an analyzer comprising:
(a) an electromagnetic radiation source for stimulating a fluorescent moiety; and
(b) an electromagnetic radiation detector that measures an electromagnetic characteristic of the fluorescent moiety when stimulated by the electromagnetic radiation.
31. The method of claim 17 , wherein step (a) further comprises assessing a non-troponin indicator in at least one sample in the series of samples, and step (b) further comprises determining a characteristic of cardiovascular health based on the concentration of the at least of one the cTnI and cTnT and the assessment of the non-troponin indicator in the at least one said sample.
32. The method of claim 31 , wherein the non-troponin indicator is at least one marker selected from the group consisting of creatine kinase (CK), myocardial fraction CK myocardial band (MB), aspartate aminotransferase, lactate dehydrogenase (LDH), α-hydroxybutyrate dehaydrogenase, myoglobin, glutamate oxaloacetate transaminase, glycogen phosphorylase BB, unbound free fatty acids, heart fatty acid binding protein (H-FABP), ischemia-modified albumin, myosin light chain 1, myosin light chain 2.
33. The method of claim 31 , wherein the non-troponin indicator is at least one marker selected from the group consisting of C-reactive protein (CRP), white blood cell count, soluble CD40 ligand, myeloperoxidase, monocyte chemoattractant protein-1, whole blood choline, and pregnancy-associated plasma protein A.
34. The method of claim 31 , wherein the non-troponin indicator is at least one marker selected from the group consisting of Interleukin 8, Interleukin 1β, Interleukin 6, Interleukin 10, Tumor Necrosis Factor, and Interleukin 12p70.
35. The method of claim 1 , wherein the antibody is attached to a fluorescent moiety.
36. The method of claim 12 , wherein electromagnetic radiation from the electromagnetic radiation source defines an interrogation space within a sample container and the detector measures the electromagnetic characteristic of the fluorescent moiety in the interrogation space.
37. The method of claim 17 , wherein the antibody is attached to a fluorescent moiety.
38. The method of claim 30 , wherein electromagnetic radiation from the electromagnetic radiation source defines an interrogation space within a sample container and the detector measures the electromagnetic characteristic of the fluorescent moiety in the interrogation space.
39. The method of claim 1 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
40. The method of claim 1 , wherein h at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnT.
41. The method of claim 17 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
42. The method of claim 17 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnT.