PATHWAY CHARACTERIZATION OF CELLS
The present invention provides methods, compositions and kits for the characterization of cellular pathways in cells containing genetic alterations.
1 . A method of classification, diagnosis, prognosis and/or prediction of an outcome of a condition in an individual, said method comprising:
a) contacting a cell population from said individual with a DNA damage or apoptosis inducing agent, wherein said cell population comprises a genetic and/or epigenetic alteration, wherein said alteration is associated with the development of said condition;
b) characterizing a plurality of DNA damage repair pathways in one or more cells from said cell population by determining an activation level of at least one activatable element within said plurality of DNA damage repair pathways;
c) determining whether said plurality of DNA damage pathways are functional in said individual based on the activation levels of said activatable elements; and
d) making a decision regarding classification, diagnosis, prognosis and/or prediction of an outcome of said condition in said individual, wherein said decision is based on said determination on step (c).
2 . The method of claim 1 further comprising performing a molecular analysis to detect said genetic alteration is said cell population.
3 . The method of claim 1 , wherein said DNA damage or apoptosis inducing agent is selected from the group consisting of Staurosporine, Etoposide, Mylotarg, Daunorubicin, Idarubicin and analogs (idarubicin, epirubicin), Ara-C, Vidaza, Mitoxantrone, Clofarabine, Cladribine, Dacogen, HydroxyUrea, Zolinza, Rituxan, Fludarabine, Floxuridine, 5-FU, Gemcitabine, Cisplatin, ifosfamide, alkylating agents, nucleoside analogs, mechlorethamine and other nitrogen mustards, mercaptopurine, temozolomide, teniposide, Thioguanine, topotecan, troxacitabine, Abraxane, Adriamycin, carboplatin, Cytoxan, Doxil, Ellence, fluorouracil, Gemzar, Ixempra, methotrexate, Mitomycin, mitoxantrone, Navelbine, Taxol, Taxotere, thiotepa, vincristine, Xeloda, Herceptin, Tykerb, Avastin, mitotic inhibitors, anti-metabolites, intercalating antibiotics, growth factor inhibitors, cell cycle inhibitors, enzymes, topoisomerase inhibitors, biological response modifiers, anti-hormones, angiogenesis inhibitors, and anti-androgens.
4 . The method of any of the preceding claims, wherein said step (c) further comprises a correlation between the activation levels of said activatable elements within said plurality of DNA damage repair pathways.
5 . The methods of claim 4 further comprising correlating the said activation levels of said activatable elements within said plurality of DNA damage repair pathways with apoptosis induced by said DNA damage or apoptosis inducing agent on said cell population.
6 . The method of any preceding claim wherein said condition is selected from the group consisting of acute leukemia, myelodysplastic syndrome and myeloproliferative neoplasms.
7 . The method of claim 6 wherein the individual has a predefined clinical parameter.
8 - 34 . (canceled)
35 . A method of classification, diagnosis, prognosis and/or prediction of an outcome of a condition in an individual, said method comprising:
a) contacting a cell population from said individual with a DNA damage or apoptosis inducing agent, wherein said cell population comprises a genetic and/or epigenetic alteration, wherein said alteration is associated with the development of said condition, and wherein said cell population is not associated and/or is not causative of said condition;
b) determining an activation level of at least one activatable element within a DNA damage pathway, an apoptosis pathway, and/or a cell cycle pathway in one or more cells from said cell population; and
c) making a decision regarding classification, diagnosis, prognosis and/or prediction of an outcome of said condition in said individual, wherein said decision is based on said activation levels of said at least one activatable element within said DNA damage pathway, an apoptosis pathway, and/or a cell cycle pathway.
36 . The method of claim 35 further comprising performing a molecular analysis to detect said genetic alteration is said cell population.
37 . The method of claim 35 , wherein said DNA damage or apoptosis inducing agent is selected from the group consisting of Staurosporine, Etoposide, Mylotarg, Daunorubicin, Idarubicin and analogs (idarubicin, epirubicin), Ara-C, Vidaza, Mitoxantrone, Clofarabine, Cladribine, Dacogen, HydroxyUrea, Zolinza, Rituxan, Fludarabine, Floxuridine, 5-FU, Gemcitabine, Cisplatin, ifosfamide, alkylating agents, nucleoside analogs, mechlorethamine and other nitrogen mustards, mercaptopurine, temozolomide, teniposide, Thioguanine, topotecan, troxacitabine, Abraxane, Adriamycin, carboplatin, Cytoxan, Doxil, Ellence, fluorouracil, Gemzar, Ixempra, methotrexate, Mitomycin, mitoxantrone, Navelbine, Taxol, Taxotere, thiotepa, vincristine, Xeloda, Herceptin, Tykerb, Avastin, mitotic inhibitors, anti-metabolites, intercalating antibiotics, growth factor inhibitors, cell cycle inhibitors, enzymes, topoisomerase inhibitors, biological response modifiers, anti-hormones, angiogenesis inhibitors, and anti-androgens.
38 . The method of claim 35 , wherein said at least one activatable element within said DNA Damage pathway is selected from the group consisting of p-Chk1, p-Chk2, p-53, p-ATM, and p-H2AX.
39 . The method of claim 35 , wherein said activatable element within said apoptosis pathway is selected from the group consisting of Cleaved PARP, Cleaved Caspase 3, Cleaved Caspase 8, BAX, Bak, and Cytochrome C.
40 . The method of claim 35 , wherein said at least one activatable element within a cell cycle pathway is selected from the group consisting of Cdc25, p-p53, cCdk1, CyclinB1, p16, p21, p-Histone H3 and Gadd45.
41 . The method of claim 35 , further comprising determining guides selection of a therapeutic treatment for said individual.
42 - 57 . (canceled)
58 . A method of determine a signaling phenotype of a cell population, wherein said cell population comprises a genetic/epigenetic alteration of interest, said method comprising:
a) subjecting said cell population comprising said genetic alteration to a plurality of modulators in separate of cultures;
b) characterizing at least one pathway in said cell population from separate plurality of cultures by determining an activation level of at least one activatable element within said at least one pathway;
c) creating a response panel for said comprising said characterization of said at least one pathway from said separate cultures; and
d) determining a signaling phenotype, wherein said signaling phenotype is based on said response panel.
59 . The method of claim 58 further comprising performing a molecular analysis to detect said genetic alteration is said cell population.
60 . The method of claim 58 , wherein said genetic alteration is a germ line alteration,
61 . The method of claim 58 , wherein said genetic alteration is an alteration in a gene selected from the group consisting of APC, AXIN2, ARF, ATM, BLM, CDH1, GPC3, CYLD, EXT1, EXT2, PTCH, SUFU, FH, SDHB, SDHC, SDHD, VHL, TP53, WT1, STK11, PTEN, TSC1, TSC2, CDKN2A, CDK4, RB1, RAD50, NF1, BMPR1A, MEN1, SMAD4, BHD, HRPT2, NF2, MUTYH, ATM, BLM, BRCA1, BRCA2, FANCA, FANCC, FANCD2, FANCE, FANCF, FANCG, NBS1, RECQL4, WRN, MSH2, MLH1, MSH6, MDM2, MRE11, NBS1, RAS, RHO, RAN, RAB, PMS2, p53, XPA, XPC, ERCC2, ERCC3, ERCC4, ERCC5, DDB2, KIT, MET, PDGFRA, RET, and DNA replication factor C.
62 . The method of claim 58 , wherein said genetic alteration in a gene from Table 1.
63 . The method of claim 58 , wherein said genetic alteration is in a BRCA gene.
64 - 101 . (canceled)