Composition for preventing or treating AIDS containing plant stem cell line derived from cambium of Panax ginseng including wild ginseng or ginseng as active ingredient
View Patent ↗The present invention relates to a composition for preventing or treating acquired immunodeficiency syndrome (AIDS), comprising one or more of the following: a homogenous cell line, and a lysate, an extract and a culture thereof as an active ingredient. The homogenous cell line, the lysate, the extract and the culture thereof, which are derived from a natural product, minimize adverse side effects of prior therapeutic agents and safe for the human body. Further, they effectively increase the count of T cells such as CD4+ T cells and decrease the number of HIVs, thereby preventing opportunistic infection, dysneuria, and neoplaia caused by immune incompetence, and ultimately the risk of death. Therefore, they are useful in preventing and treating AIDS, and relieving symptoms of AIDS.
1. A method of ameliorating acquired immunodeficiency syndrome (AIDS), the method comprising administering a cell line derived from the cambium of Panax ginseng , wherein the cell line has the following characteristics:
(a) it is in an innately undifferentiated state; and
(b) it is a homogeneous cell line.
2. The method of claim 1 , wherein the cell line further has the following characteristics:
(a) it is present as single cell level during a suspension culture;
(b) it has a lower sensitivity to shear stress in a bioreactor, compared to cell lines derived from tissues other than the cambium of Panax ginseng ; and
(c) its growth rate is higher than those of cell lines derived from tissues other than the cambium of Panax ginseng and is cultured stably.
3. The method of claim 1 , wherein the cell line is obtained by using an isolation method comprising the steps of:
(a) obtaining a Panax ginseng cambium-containing tissue;
(b) culturing the cambium-containing tissue in a medium supplemented with IAA (indole-3-acetic acid) or IBA (indole-3-butyric acid) to induce a cell line derived from the cambium, wherein an osmotic stress is applied to the cambium-containing tissue before, during, or after the culture; and
(c) collecting the induced, cambium-derived cell line.
4. The method of claim 3 , wherein step (c) is performed by proliferating the induced cambium-derived cell line in a medium which contains one or more of 2,4-D (2,4-dichlorophenoxyacetic acid), picloram and IBA, and then collecting the cambium-derived cells.
5. A method of ameliorating AIDS, the method comprising orally administering functional food comprising a cell line derived from the cambium of Panax ginseng , wherein the cell line has the following characteristics:
(a) it is in an innately undifferentiated state; and
(b) it is a homogeneous cell line.
6. The method of claim 5 , wherein the cell line further has the following characteristics:
(a) it is present as single cell level during a suspension culture;
(b) it has a lower sensitivity to shear stress in a bioreactor, compared to cell lines derived from tissues other than the cambium of Panax ginseng ; and
(c) its growth rate is higher than those of cell lines derived from tissues other than the cambium of Panax ginseng and is cultured stably.
7. The method of claim 5 , wherein the cell line is obtained by using an isolation method comprising the steps of:
(a) obtaining a Panax ginseng cambium-containing tissue;
(b) culturing the cambium-containing tissue in a medium supplemented with IAA or IBA to induce a cell line derived from the cambium, wherein an osmotic stress is applied to the cambium-containing tissue before, during, or after the culture; and
(c) collecting the induced, cambium-derived cell line.
8. The method of claim 7 , wherein step (c) is performed by proliferating the induced cambium-derived cell line in a medium which contains one or more of 2,4-D (2,4-dichlorophenoxyacetic acid), picloram and IBA, and then collecting the cambium-derived cells.
9. A method of increasing T cells, the method comprising administering a cell line, derived from the cambium of Panax ginseng , wherein the cell line and has the following characteristics:
(a) it is in an innately undifferentiated state; and
(b) it is a homogeneous cell line.
10. The method of claim 9 , wherein the cell line is obtained by using an isolation method comprising the steps of:
(a) obtaining a Panax ginseng cambium-containing tissue;
(b) culturing the cambium-containing tissue in a medium supplemented with IAA or IBA to induce a cell line derived from the cambium, wherein an osmotic stress is applied to the cambium-containing tissue before, during, or after the culture; and
(c) collecting the induced, cambium-derived cell line.
11. The method of claim 10 , wherein step (c) is performed by proliferating the induced cambium-derived cell line in a medium which contains one or more of 2,4-D (2,4-dichlorophenoxyacetic acid), picloram and IBA, and then collecting the cambium-derived cells.