Method for detecting nucleic acid amplification in sample and device therefor
View Patent ↗A device for detecting nucleic acid amplification in a sample, comprising: a loading unit (thermoregulation unit) which is provided with a plurality of holes for loading reaction containers and capable of arbitrarily controlling a measurement unit for measuring samples in the containers that are loaded in the holes. The loading unit and the measurement unit, which are placed opposite to each other, can be operated independently from each other. In a transfer operation, the operation speeds of the thermoregulation unit and measurement unit are controlled so that the sum of these speeds amounts to an arbitrary constant value.
1. A method for detecting nucleic acid amplification, the method comprising:
(a) loading a new reaction container comprising a sample and a reagent for nucleic acid amplification into an empty loading position in a loading unit;
(b) simultaneously measuring with a measurement unit a nucleic acid amplification reaction in reaction containers previously loaded into loading positions of the loading unit;
(c) performing a rotational movement or linear movement of the measurement unit in a predetermined direction relative to the loading unit to sequentially receive a measurement signal from each sample in reaction containers located in the loading unit in a fixed period;
wherein loading the new reaction container does not interrupt measuring the nucleic acid amplification reaction in reaction containers previously loaded into loading positions of the loading unit;
wherein the loading unit has a thermoregulation unit;
wherein the measurement unit faces the loading unit;
wherein each of the loading unit and the measurement unit has an independent movement mechanism;
wherein a cover covers the loading unit and comprises a reaction-container loading gate;
wherein the measurement unit rotation velocity is fixed while the loading unit is making a stop; and
wherein the measurement unit maintains a relative velocity that is constant with respect to a loading unit rotation velocity when an empty position of the loading unit is moved to the reaction-container loading gate.
2. The method according to claim 1 , wherein the thermoregulation unit of the loading unit is set to be controlled by an independent temperature program for each of reaction containers or reaction container groups to be loaded.
3. The method according to claim 1 , wherein the thermoregulation unit of the loading unit is set to perform constant temperature control in nucleic acid sequence-based amplification.
4. The method according to claim 1 , further comprising:
setting a temporal range to receive measurement signals from the reaction containers, wherein the temporal range covers a plurality of specific measurement ranges with different time periods which depend on a time period for detecting various samples, and
setting a measurement-signal receiving period within the temporal range to receive measurement signals, wherein the measurement-signal receiving period covers all the specific measurement ranges by controlling the relative velocity between the loading unit and the measurement unit, and
selecting a specific measurement range for nucleic-acid amplification detection from the temporal range to receive measurement signals for each reaction container or reaction container group to be loaded into the loading unit, and
extracting a measurement signal contained in the selected specific measurement range as measurement data for nucleic-acid amplification detection.