IP Library › Granted Patent US 9,512,199
Granted Patent B2
US 9,512,199 · App. 13/813,409 · Granted Dec 6, 2016

Fibronectin cradle molecules and libraries thereof

Inventors: Andreas Loew (Somerville, MA); Brian Edward Vash (Cambridge, MA); Shohei Koide (Chicago, IL); John Bernard Wojcik (Chicago, IL); Akiko Koide (Chicago, IL); Ryan Nicholas Gilbreth (Chicago, IL)
Assignees: NOVARTIS AG; UNIVERSITY OF CHICAGO
C07K14/78C07K16/18C07K16/40C07K16/42C07K2317/34C07K2318/20
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Quick Facts
Patent No.
US 9,512,199
App. No.
13/813,409
Granted
Dec 6, 2016
Kind
B2
Abstract

The here described invention discloses a combination of a top and bottom loop binder library using the CD and the FG loops of a number of FnIII domains (FnIII) (e.g., FnIII 7 , FnIII 10 and FnIII 14 ) together with the surface exposed residues of the beta-sheet. The invention also pertains to a method of forming a library of FnIII domain polypeptides useful in screening for the presence of one or more polypeptides having a selected binding or enzymatic activity.

Claims (5)

1. A 10 th fibronectin type III (FnIII10) domain-based cradle polypeptide capable of binding to a target molecule, wherein the cradle polypeptide comprises the sequence of SEQ ID NO: 44.

2. A molecule comprising two or more cradle polypeptides of claim 1 linked together.

3. A molecule of claim 2 , wherein each of the cradle polypeptides binds to the same target molecule.

4. A molecule of claim 2 , wherein two or more of the cradle polypeptides bind to different target molecules.

5. A cradle library comprising a plurality of cradle polypeptides of claim 1 .

Assignments (4)
CONFIRMATORY LICENSE Recorded Sep 1, 2020
From: UNIVERSITY OF CHICAGO
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 053656/0705 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 7, 2013
From: VASH, BRIAN EDWARD; LOEW, ANDREAS
To: NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH INC.
Reel/Frame 031564/0711 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 7, 2013
From: NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH INC.
To: NOVARTIS AG
Reel/Frame 031564/0729 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 7, 2013
From: KOIDE, SHOHEI; WOJCIK, JOHN BERNARD; KOIDE, AKIKO; GILBRETH, RYAN NICHOLAS
To: THE UNIVERSITY OF CHICAGO
Reel/Frame 031564/0733 →
Continuity (6)
Provisional Application 61369160 · Jul 30, 2010
Provisional Application 61369203 · Jul 30, 2010
Provisional Application 61369222 · Jul 30, 2010
Provisional Application 61474632 · Apr 12, 2011
Provisional Application 61474648 · Apr 12, 2011
Related Publication 20140057807A1 · Feb 27, 2014