Methods of generating oligodendrocytes and cell populations comprising same
A method of generating a population of cells useful for treating a brain disorder in a subject is disclosed. The method comprises contacting mesenchymal stem cells (MSCs) with at least one exogenous miRNA having a nucleic acid sequence at least 90% identical to a sequence selected from the group consisting of SEQ ID NOs: 15-19 and 27-35, thereby generating the population of cells and/or generating neurotrophic factors that may provide important signals to damaged tissues or locally residing stem cells. MSCs differentiated by miRs may also secrete miRs and deliver them to adjacent cells and therefore provide important signals to neighboring endogenous normal or malignant cells.
1. A method of treating a brain tumor in a subject in need thereof, the method comprising transplanting a therapeutically effective amount of mesenchymal stem cells which have been modified to express exogenous miRNA miR-124, wherein said mesenchymal stem cells deliver said exogenous miRNA to the cytosol of a cell of the tumor, thereby treating the brain tumor.
2. The method of claim 1 , wherein said brain tumor is a glioma.
3. The method of claim 1 , further comprising expressing in said mesenchymal stem cells a pro-apoptotic agent.
4. The method of claim 3 , wherein said pro-apoptotic agent comprises soluble TNF-related apoptosis-inducing ligand (sTRAIL).