Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having cellobiohydrolase activity comprises:
an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.
2. A recombinant host cell comprising the nucleic acid construct of claim 1 .
3. A method of producing a polypeptide having cellobiohydrolase activity, said method comprising:
(a) cultivating the host cell of claim 2 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
4. A transgenic plant, plant part or plant cell transformed with the nucleic acid construct of claim 1 .
5. A method of producing a polypeptide having cellobiohydrolase activity, said method comprising:
(a) cultivating the transgenic plant or plant cell of claim 4 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
6. A nucleic acid construct or expression vector comprising a gene encoding a protein,
wherein the gene is operably linked to a polynucleotide that encodes a signal peptide comprising amino acids 1 to 17 of SEQ ID NO: 2, and
wherein the gene is foreign to the polynucleotide encoding the signal peptide.
7. An isolated recombinant host cell comprising the nucleic acid construct or expression vector of claim 6 .
8. A process of producing a protein, said process comprising:
(a) cultivating the recombinant host cell of claim 7 under conditions conducive for production of the protein; and
(b) recovering the protein.
9. A process for degrading or converting a cellulosic material, said process comprising:
(i) treating the cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having cellobiohydrolase activity; and
(ii) recovering the degraded or converted material;
wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.
10. A process for producing a fermentation product, said process comprising:
(a) saccharifying a cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having cellobiohydrolase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation;
wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.
11. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 97% sequence identity to mature polypeptide of SEQ ID NO: 2.
12. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 98% sequence identity to mature polypeptide of SEQ ID NO: 2.
13. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 99% sequence identity to mature polypeptide of SEQ ID NO: 2.
14. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises the amino acid sequence of SEQ ID NO: 2.
15. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having amino acids 18 to 450 of SEQ ID NO: 2.
16. A recombinant expression vector comprising the nucleic acid construct of claim 1 .
17. The process of claim 9 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 97% sequence identity to mature polypeptide of SEQ ID NO: 2.
18. The process of claim 9 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having amino acids 18 to 450 of SEQ ID NO: 2.
19. The process of claim 10 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 97% sequence identity to mature polypeptide of SEQ ID NO: 2.
20. The process of claim 10 , wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having amino acids 18 to 450 of SEQ ID NO: 2.
21. An isolated recombinant host cell transformed with a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polynucleotide is heterologous to the recombinant host cell, and wherein the polypeptide having cellobiohydrolase activity comprises an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.