DRIED HYDROGEL, DRIED VITRIGEL MEMBRANE, AND METHODS FOR PRODUCING THE SAME
A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.
1 . A dried vitrigel membrane, characterized by not having on amorphous outer peripheral edge.
2 . The dried vitrigel membrane according to claim 1 , characterized by attaching to a substrate.
3 . The dried vitrigel membrane according to claim 1 , characterized in that the dried vitrigel membrane is superposed with a film possessing a capacity facilitating the detachability of the dried vitrigel membrane.
4 . The dried vitrigel membrane according to claim 3 , characterized in that the film is Parafilm.
5 . A dried hydrogel, characterized by not having, an amorphous outer peripheral edge.
6 . The dried hydrogel according to claim 5 , characterized by attaching to a substrate.
7 . The dried hydrogel according to claim 5 , characterized in that the dried hydrogel is superposed with a film possessing a capacity facilitating the detachability of the dried hydrogel.
8 . The dried hydrogel according to claim 7 , characterized in that the film is Parafilm.
9 . A method for producing a dried vitrigel membrane having a desired shape, characterized by including the following steps of:
(1) a step of keeping a hydrogel in the inside of a well surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of a free water within the hydrogel from a gap between the substrata and the wall surface mold;
(2) a step of removing the wall surface mold from the top of the substrate;
(3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel;
(4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and
(5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.
10 . The method for producing a dried vitrigel membrane according to claim 9 , characterized in that in the step (1), a support is introduced into the hydrogel in the inside of the wall surface mold.
11 . The method for producing a dried vitrigel membrane according to claim 9 , characterized in that in the step (1), the amount of the free water within the hydrogel is reduced to from about ¼ to ¾.
12 . The method for producing a dried vitrigel membrane according to claim 9 , characterized in that in the step (5), the dried vitrigel membrane is vitrified on a film possessing a capacity facilitating the detachability of the dried vitrigel membrane.
13 . The method for producing a dried vitrigel membrane according to claim 12 , characterized in that the film is Parafilm.
14 . A method for producing a dried vitrigel membrane capable of being molded in a desired shape, characterized by including the following steps of:
(1) a step of keeping a hydrogel in the inside of a surface wall mold having an arbitrary shape, disposed on a substrate on which a film possessing a capacity facilitating the detachability of the dried vitrigel membrane is laid, and discharging a part of free water within the hydrogel from a gap between the film on the substrate and the wall surface mold;
(2) a step of removing the wall surface mold from the top of the substrate;
(3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hyrogel;
(4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and
(5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.
15 . The method for producing a dried vitrigel membrane according to claim 14 , characterized by after the step (5), including
(6) a step of cutting the dried vitrigel membrane superposed with the film in a desired shape.
16 . The method for producing a dried vitrigel membrane according to claim 15 , characterized by after the step (6), including
a step of releasing the dried vitrigel membrane from the film.
17 . The method for producing a dried vitrigel membrane according to claim 14 , characterized in that the film is Parafilm.
18 . The method for producing a dried vitrigel membrane according to claim 14 , characterized in that in the step (1), a support is introduced into the hydrogel in the inside of the wall surface mold.
19 . The method for producing a dried vitrigel membrane according to claim 14 , characterized in that in the step (1), the amount of the free water within the hydrogel is reduced to from about ¼ to ¾.
20 . A method for producing a dried hydrogel having a desired shape, characterized by including the following steps of:
(1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of a free water within the hydrogel from a gap between the substrate and the wall surface mold;
(2) a step of removing the wall surface mold from the top of the substrate; and
(3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel.
21 . The method for producing a dried hydrogel according to claim 20 , characterized in that the step (1), a support is introduced into the hydrogel in the inside of the wall surface mold.
22 . The method for producing a dried hydrogel according to claim 20 , characterized in that in the step (1), the amount of the free water within the hydrogel is reduced to from about ¼ to ¾.
23 . The method for producing a dried hydrogel according to claim 20 , characterized in that in the step (3), the vitrification is conducted on a film possessing a capacity facilitating the detachability of the dried hydrogel.
24 . The method for producing a dried hydrogel according to claim 23 , characterized in that the film is Parafilm.
25 . A method for producing a dried hydrogel capable of being molded in a desired shape, characterized by including the following steps of:
(1) a step of keeping a hydrogel in the inside of a surface wall mold having an arbitrary shape, disposed on a substrate on which a film possessing a capacity facilitating the detachability of the dried hydrogel is laid, and discharging a part of free water within the hydrogel from a gap between the film on the substrate and the wall surface mold;
(2) a step of removing the wall surface mold from the top of the substrate; and
(3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel.
26 . The method for producing a dried hydrogel according to claim 25 , characterized by after the step (3), including
(4) a step of cutting the dried hydrogel superposed with the film in a desired shape.
27 . The method for producing a dried hydrogel seconding to claim 26 , characterized by offer the step (4), including
a step of releasing the dried hydrogel from the film.
28 . The method for producing a dried hydrogel according to claim 25 , characterized in that in the step (1), a support is introduced into the hydrogel to the inside of the wall surface mold.
29 . The method for producing a dried hydrogel according to claim 25 , characterized in that in the step (1), the amount of the free water within the hydrogel is reduced to from about ¼ to ¾.
30 . The method for producing a dried hydrogel according to claim 25 , characterized in that the film is Parafilm.
31 . A laminate, characterized in that the dried vitrigel membranes according to claim 1 are superposed and integrated.
32 . An equivalent of collagen constituting mainly a substance of a cornea using a laminate obtained by superposing plural sheets of vitrigel membranes in which the dried vitrigel membrane according to claim 1 is made of collagen as a raw material, and this dried vitrigel membrane is rehydrated.
33 . An equivalent of collagen constituting mainly a substance of a cornea using a vitrigel membrane obtained by rehydrating a laminate in which the dried vitrigel membrane according to claim 1 is made of collagen as a raw material, and these dried vitrigel membranes are superposed and integrated.