Maturation of gastrointestinal tract
The present invention provides a method to induce maturation of an immature GI-tract, such as intestine, e.g. small intestine, the method comprising the steps of administering a mixture of enzymes to the immature GI-tract, said enzymes having a pancreatic activity or action, and/or pancreatic like activity or action, and analyzing the maturation process of the GI-tract to monitor said maturation process. Provided herein are also uses and kits to provide for GI-tract maturation.
1. A method of treating a subject having an immature GI-tract, the method comprising:
administering a mixture comprising about 25,000-500,000 USP/kg bodyweight per day of lipase, about 37,500-1,250,000 USP/kg bodyweight per day of protease, and about 58,100 to 2,490,000 USP/kg bodyweight per day of amylase to a subject in need thereof, analysing a biological sample collected from said subject for maturation of the GI-tract, wherein at least one of intestinal enterocyte morphology or intestinal enterocyte biomarkers is analysed to monitor said maturation.
2. The method of claim 1 , wherein the immature GI-tract is in a newborn human.
3. The method of claim 1 , wherein a change in at least one of intestinal enterocyte morphology, intestinal enterocyte biomarkers, and weight of the intestine is indicative of GI-tract maturation, wherein an increase of the weight of the intestine is indicative of maturation.
4. The method of claim 1 , wherein the mixture of enzymes is administered orally.
5. A method of treating an immature GI-tract disorder in a subject in need thereof, wherein the immature GI-tract disorder is necrotizing enterocolitis and wherein the method comprises administering to the subject a mixture comprising about 25,000-500,000 USP/kg bodyweight per day of lipase, about 37,500-1,250,000 USP/kg bodyweight per day of protease, and about 58,100 to 2,490,000 USP/kg bodyweight per day of amylase to induce GI-tract maturation.
6. The method of claim 5 , wherein the subject in the need thereof is an infant.