Kits for detecting breast or ovarian cancer in a body fluid sample and use thereof
The present invention relates to a method for the accurate, rapid and sensitive detection of breast or ovarian cancers from body fluid samples of a mammalian subject and related assay, kits and peptides suitable for such a method.
1. A kit for detecting one or more autoantibodies associated with a breast cancer or an ovarian cancer in a biological fluid sample of a mammalian subject, the kit comprising a peptide or a combination of peptides coupled to a solid support matrix, wherein the peptide or combination of peptides comprises:
a) a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5;
b) a combination of peptides comprising a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5 and a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 1, a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 4 and a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 12; or
c) a combination of peptides comprising 1) a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5 and 2) at least one peptide consisting of the amino acid set forth in SEQ ID NO: 1 SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12 or a combination thereof,
wherein the one or more autoantibodies bind to one or more of the peptides comprised in the kit.
2. The kit of claim 1 , wherein the peptide is a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5.
3. The kit of claim 1 , wherein said kit comprises a combination of peptides comprising
a) a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5; and
b) at least one peptide consisting of the amino acid sequence set forth in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12 or a combination thereof.
4. The kit of claim 1 , wherein said kit comprises a combination of peptides comprising a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5, a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 1, a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 4 and a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 12.
5. A method for detecting a breast or an ovarian cancer from a biological fluid sample of a mammalian subject comprising the steps of:
(a) providing a biological fluid sample from a mammalian subject;
(b) bringing said biological fluid sample into contact with the solid matrix of the kit of claim 1 , wherein the contacting is under conditions sufficient for binding an antibody present in said biological fluid sample to said solid matrix through antigen-antibody interactions;
(c) removing the biological fluid sample from the solid matrix to remove unbound antibody from the surface of said solid matrix; and
(d) detecting the presence of an antigen-antibody complex bound to said solid matrix,
wherein the presence of said complex is indicative that the biological fluid sample contains one or more breast or ovarian-cancer associated-endogenous antibodies.
6. The method according to claim 5 , wherein the peptide bound to the solid matrix is a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5.
7. The method according to claim 5 , wherein the combination of peptides bound to the solid matrix comprises a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5 and a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 1, a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 4 and a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 12.
8. The method according to claim 5 , wherein the combination of peptides bound to the solid matrix comprises 1) a peptide consisting of the amino acid sequence set forth in SEQ ID NO: 5 and 2) at least one peptide consisting of the amino acid set forth in SEQ ID NO: 1 SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12 or a combination thereof.