Bicarbonate trigger for inducing lipid accumulation in algal systems
The present invention provides bicarbonate containing and/or bicarbonate-producing compositions and methods to induce lipid accumulation in an algae growth system, wherein the algae growth system is under light-dark cycling condition. By adding said compositions at a specific growth stage, said methods lead to much higher lipid accumulation and/or significantly reduced total time required for accumulating lipid in the algae growth system.
1. A method of inducing lipid accumulation in an algae growth system, wherein the algae growth system is under light-dark cycling, said method comprising adding a triggering composition when algae cells in the growth system are in exponential growth stage, but prior to nutrient depletion of the growth system, wherein said triggering composition comprises bicarbonate, and/or one or more compounds that provide bicarbonate after the composition is added into the algae growth system, and wherein algae replication in the growth system is inhibited at the time when the triggering composition is added.
2. The method of claim 1 , wherein the inhibition happens during late phase of the exponential growth of algae in the system.
3. The method of claim 1 , wherein the triggering composition further comprises one or more agents that increase the pH of the algae growth system.
4. The method of claim 1 , wherein the inhibition comprises an aeration shift of CO 2 concentration in the growth system from high to low, wherein the shift is sufficient to inhibit algae replication.
5. The method of claim 1 , wherein the algae is a Scenedesmus sp., a Chlorophyta sp. or a diatom.
6. The method of claim 1 , wherein the triggering composition increases the pH of the algae growth system to at least pH 9.5.
7. The method of claim 1 , wherein the triggering composition provides the algae growth system a concentration of bicarbonate of at least 10 mM.
8. The method of claim 1 , wherein the triggering composition is added into the growth system as close to nutrient depletion of the growth system as possible.
9. The method of claim 1 , wherein the lipid is triacylglycerol (TAG).
10. The method of claim 1 , wherein the lipid accumulation in the algae growth system is at least 5 times the lipid accumulation in a control algae growth system in which said triggering composition is not added.