Plant regulatory elements and uses thereof
The present invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants. Transgenic plants, plant cells, plant parts, and seeds comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.
1. A DNA molecule comprising the DNA sequence of SEQ ID NO: 2, wherein said sequence is operably linked to heterologous transcribable polynucleotide molecule.
2. The DNA molecule of claim 1 , wherein said sequence comprises SEQ ID NO:3.
3. The DNA molecule of claim 1 , wherein the heterologous transcribable polynucleotide molecule comprises a gene of agronomic interest.
4. The DNA molecule of claim 3 , wherein the gene of agronomic interest confers herbicide tolerance in plants.
5. The DNA molecule of claim 3 , wherein the gene of agronomic interest confers pest resistance in plants.
6. A transgenic plant cell comprising a heterologous DNA molecule comprising the sequence of SEQ ID NO: 2, wherein said sequence is operably linked to heterologous transcribable polynucleotide molecule.
7. The transgenic plant cell of claim 6 , wherein said transgenic plant cell is a monocotyledonous plant cell.
8. The transgenic plant cell of claim 6 , wherein said transgenic plant cell is a dicotyledonous plant cell.
9. A transgenic plant, or part thereof, comprising the DNA molecule of claim 1 .
10. A progeny plant of the transgenic plant of claim 9 , or a part thereof, wherein the progeny plant or part thereof comprises said DNA molecule.
11. A transgenic seed, wherein the seed comprises the DNA molecule of claim 1 .
12. A transgene cassette comprising a transcriptional regulatory expression element selected from the group consisting of SEQ ID NOs: 1, 6, and 8, wherein the transcription regulatory element is operably linked to a heterologous coding sequence that is operably linked to a 3′UTR selected from the group consisting of SEQ ID NOs: 10, 11, 12, and 13.
13. The transgene cassette of claim 12 , wherein the transcription regulatory element is SEQ ID NO: 1, and wherein the 3′UTR is SEQ ID NO: 10.
14. The transgene cassette of claim 12 , wherein the transcription regulatory element is SEQ ID NO: 6, and wherein the 3′UTR is selected from the group consisting of SEQ ID NOs: 11 and 12.
15. The transgene cassette of claim 12 , wherein the transcription regulatory element is SEQ ID NO: 8, and wherein the 3′UTR is selected from the group consisting of SEQ ID NOs: 12 and 13.
16. A method of producing a commodity product comprising obtaining a transgenic plant or part thereof according to claim 9 and producing the commodity product therefrom.
17. The method of claim 16 , wherein the commodity product is protein concentrate, protein isolate, grain, starch, seeds, meal, flour, biomass, or seed oil.
18. A method of expressing a transcribable polynucleotide comprising cultivating the plant of claim 9 , wherein the transcribable polynucleotide is expressed.
19. The transgenic plant cell of claim 6 , wherein said sequence comprises SEQ ID NO: 3.