SUBSTRATES FOR GENERATION OF CONTROLLED HUMAN PLURIPOTENT STEM CELL COLONY SIZE AND SHAPE
Methods of cell culture using patterned SAM arrays are disclosed. Advantageously, the disclosed methods use SAM arrays presenting adhesion peptides to grow confluent monolayers that can invaginate to form an embryoid body.
1 . A method of controlling the formation of a cell culture aggregate, the method comprising: culturing a cell on an array spot of a self-assembled monolayer array for a sufficient time to form a confluent monolayer of cells; and detaching the confluent monolayer of cells.
2 . The method of claim 1 , wherein the confluent monolayer is mechanically detached from the array spot.
3 . The method of claim 1 , wherein the confluent monolayer is cultured until the confluent monolayer spontaneously detaches from the array spot.
4 . The method of claim 3 , wherein the confluent monolayer is cultured for a period of from about 36 hours to about 84 hours.
5 . The method of claim 1 , further comprising culturing the confluent monolayer for a sufficient time to allow the confluent monolayer to invaginate.
6 . The method of claim 5 , wherein the confluent monolayer is cultured for a period of from about 10 hours to about 72 hours.
7 . The method of claim 1 , wherein the cell is selected from the group consisting of an induced pluripotent stem cell, a mesenchymal stem cell, an umbilical vein endothelial cell, a dermal fibroblast, a fibrosarcoma cell, an embryonic stem cell, an iPS IMR90-4 cell and combinations thereof.
8 . A method of preparing a cell aggregate of a uniform size, the method comprising:
culturing a cell on an array spot of a self-assembled monolayer array spot of a specified diameter for a sufficient time to form a confluent monolayer of cells;
detaching the confluent monolayer of cells; and
collecting the confluent monolayer of cells.
9 . The method of claim 8 , wherein the specified diameter of the array spot is from about 600 μm to about 6 mm.
10 . The method of claim 8 , further comprising culturing the confluent monolayer for a sufficient time to allow the confluent monolayer to invaginate.
11 . The method of claim 8 , wherein the confluent monolayer is mechanically detached from the array spot.
12 . The method of claim 8 , wherein the confluent monolayer is cultured until the confluent monolayer spontaneously detaches from the array spot.
13 . The method of claim 8 , wherein the cell is selected from the group consisting of an induced pluripotent stem cell, a mesenchymal stem cell, an umbilical vein endothelial cell, a dermal fibroblast, a fibrosarcoma cell, an embryonic stem cell, an iPS IMR90-4 cell and combinations thereof.
14 . A method of preparing a cell aggregate of a specified shape, the method comprising:
culturing a cell on a self-assembled monolayer array spot of a specified shape for a sufficient time to form a confluent monolayer of cells;
detaching the confluent monolayer of cells; and
collecting the confluent monolayer of cells.
15 . The method of claim 14 , wherein the specified shape is selected from the group consisting of a circle, an oval, an oval cross, a star, and a hand.
16 . The method of claim 14 , further comprising culturing the confluent monolayer for a sufficient time to allow the confluent monolayer to invaginate.
17 . The method of claim 14 , wherein the confluent monolayer is mechanically detached from the array spot.
18 . The method of claim 14 , wherein the confluent monolayer is cultured until the confluent monolayer spontaneously detaches from the array spot.
19 . The method of claim 14 , wherein the cell is selected from the group consisting of an induced pluripotent stem cell, a mesenchymal stem cell, an umbilical vein endothelial cell, a dermal fibroblast, a fibrosarcoma cell, an embryonic stem cell, an iPS IMR90-4 cell and combinations thereof.