Compositions, methods and kits for therapeutic treatment with wet spun microstructures
Methods, compositions, systems, devices and kits are provided for preparing and using a multi-layer polymeric microstructure composition for delivering a therapeutic agent to a subject. In various embodiments, the therapeutic agent includes at least one selected from the group of: a drug, a protein, a sugar, a carbohydrate, and a nucleotide sequence. In related embodiments, the composition is a fiber, a suture, a sphere, an implant, or a scaffold.
1. A wet spun microfiber composition comprising at least one polymer wherein the composition comprises:
a porous polymeric microstructure obtained by solvent induced crystallization (SINC), wherein the polymer is at least one selected from: poly-1-lactic acid (PLLA), poly-lactic-co-glycolide (PLGA), polyvinylpyrrolidone (PVP); and further comprises at least one encapsulated therapeutic agent, the polymer is dissolved in an organic solvent and the therapeutic agent is dispersed with the polymer in the organic solvent to produce a polymer-therapeutic agent solution, and the polymer-therapeutic agent solution is wet spun by extrusion in a non-solvent bath, provided that the non-solvent bath is not water, to obtain the wet spun microstructure; wherein the therapeutic agent is located in the microstructure and is controllably releasable from the composition, and wherein following evaporation of the solvent and the non-solvent the microfiber composition has a degree of crystallinity at least 10% greater than that of control polymer prior to wet spinning.
2. The composition according to claim 1 having a structure selected from the group of: a fiber, a suture, a sphere, an implant, and a scaffold.
3. The composition according to claim 2 , wherein an encapsulated first therapeutic agent comprises dexamethasone.
4. The composition according to claim 3 , further comprising an encapsulated second therapeutic agent.
5. The composition according to claim 4 , wherein the second therapeutic agent comprises at least one selected from the group: a drug; a protein; a peptide; a sugar; a carbohydrate; and a nucleotide sequence.
6. The composition according to claim 5 , wherein the protein comprises at least one selected from the group: a growth factor, an immunoglobulin, an enzyme, and a peptide antibiotic.
7. The composition according to claim 5 , wherein the nucleotide sequence comprises a vector.
8. The composition according to claim 4 , wherein the second therapeutic agent is a Nog (Noggin).
9. The microfiber composition according to claim 1 , wherein the composition comprises at least about 75% of the initial tensile strength for at least about five weeks.
10. A kit for treating a subject having a medical condition comprising:
a wet spun microfiber composition having at least one polymer selected from: poly-1-lactic acid (PLLA), poly-lactic-co-glycolide (PLGA), polyvinylpyrrolidone (PVP); wherein the composition comprises a porous polymeric microstructure obtained by solvent induced crystallization (SINC), and further comprises at least one encapsulated therapeutic agent, the polymer is dissolved in an organic solvent and the therapeutic agent is dispersed with the polymer in the organic solvent to produce a polymer-therapeutic agent solution, and the polymer-therapeutic agent solution is wet spun by extrusion in a non-solvent bath, provided that the non-solvent bath is not water, to obtain the wet spun microstructure; wherein the therapeutic agent is located in the microstructure and is controllably releasable from the composition, and wherein the microfiber composition has a degree of crystallinity at least 10% greater than that of control polymer prior to wet spinning;
instructions for use; and,
a container.
11. The kit according to claim 10 wherein a first therapeutic agent is dexamethasone.
12. The kit according to claim 10 further comprising a second therapeutic agent.
13. The kit according to claim 12 , wherein the second therapeutic agent comprises at least one selected from the group: a sugar; a carbohydrate; a nucleotide sequence; a protein selected from the group of: a growth factor, an immunoglobulin, an enzyme, and an antibiotic; and a drug selected from the group comprising: an anti-apoptotic; an immunosuppressant; a pro-apoptotic; an anti-coagulant; an anti-tumor; an anti-viral; an anti-bacterial; an anti-mycobacterial; an anti-fungal; an anti-proliferative; an anti-inflammatory; and a steroid selected from the group of: a cortisone compound, a dexamethasone, a sex-related hormone; and a non-steroidal anti-inflammatory agent (NSAID).
14. The kit according to claim 13 , wherein the nucleotide sequences comprises a vector.
15. The kit according to claim 10 wherein the composition has a structure selected from the group of: a fiber, a suture, a sphere, an implant, and a scaffold.
16. A device for treating a tissue comprising:
at least one microstructure polymer obtained by solvent induced crystallization (SINC), and a composition, wherein the polymer secures or binds the tissue and is selected from the group of: a suture, a strand, a fiber, a filament, and a thread; wherein the polymer is a biocompatible ester compound and is at least one selected from: poly-1-lactic acid (PLLA), poly-lactic-co-glycolide (PLGA), polyvinylpyrrolidone (PVP); and the composition contains at least one therapeutic agent that forms a complex with the polymer; wherein the polymer is dissolved in an organic solvent and the therapeutic agent is dispersed in with the polymer in the organic solvent to produce a polymer-therapeutic agent solution, and the polymer-therapeutic agent solution is wet spun by extrusion in a non-solvent bath, provided that the non-solvent bath is not water, resulting in the wet spun microstructure; and is characterized by controllable release from the polymer.
17. The device according to claim 16 wherein the polymer comprises plurality of polymers.
18. The device according to claim 17 wherein the plurality of polymers is interlinked or bound closely together, wherein the plurality of polymers forms a structure selected from the group of: a screen, a fabric, a scaffold, a yarn, an implant, and a mesh.
19. The device according to claim 16 , wherein the polymer is further characterized by at least one property selected from the group of: crystalline, amorphous, bio-resorbable, porous, elastic, and sterile.
20. The device according to claim 16 , wherein the composition further comprises an additional agent that modulates strength or elasticity of the polymer, or that modulates release of the therapeutic agent from the device.
21. The device according to claim 20 , wherein the polymer comprises a water-soluble polymer.
22. The device according to claim 21 , wherein the polymer comprises an internal structure that is crystalline, amorphous, or a combination thereof.
23. The device according to claim 22 , wherein the therapeutic agent comprises at least one of the group selected from: a low molecular weight drug, a glucosteroid, a steroid hormone, a protein, a peptide, a sugar, a carbohydrate, and a nucleotide sequence.
24. The device according to claim 22 , wherein the therapeutic agent comprises at least one selected from the group of: a lysozyme, an insulin, dexamethasone, a noggin.
25. The device according to claim 22 , wherein the therapeutic agent is at least one selected from the group consisting of: an anti-tumor, an antiviral, an antibacterial, an anti-inflammatory, an anti-mycobacterial, an anti-fungal, an anti-proliferative, an anti-apoptotic, and a bone morphogenic protein antagonist.
26. The device according to claim 25 , wherein the polymer contacts the tissue and releases the therapeutic agent and treats or remediates a defect or a condition of cells of the tissue, wherein the tissue is selected from the group of: epithelial, endothelial, vascular, nerve, muscle, cartilage, and bone.