Polysilicate-polysilicone enzyme immobilization materials
The present invention generally relates to improvements in enzyme immobilization, particularly for use in the field of carbon dioxide capture and sequestering. It has been discovered that the utilization of sol-gel processes to immobilize enzymes in polysilicate-polysilicone copolymer coatings and particles, and the deposition of these coatings on solid state supports or use of suspensions of these particles, provides significant benefits for use in industrial applications involving enzymatic catalysts.
1. A coated support comprising a polysilicate-polysilicone copolymer immobilizing a biocatalyst; the polysilicate-polysilicone copolymer adhered to a solid support by an adhesive coating and wherein the biocatalyst comprises a carbonic anhydrase.
2. A coated support comprising
a solid support;
a coating composition forming a layer on the surface of the solid support, the coating composition comprising a polysilicate-polysilicone copolymer and a hydrophilic additive; and
a biocatalyst that catalyzes hydration of carbon dioxide being entrapped in the coating composition;
wherein the biocatalyst comprises a carbonic anhydrase.
3. The coated support of claim 1 wherein the adhesive coating comprises a polymer adhesive.
4. The coated support of claim 3 wherein the polymer adhesive comprises a urethane polymer, an epoxy polymer, a resin, a cyanoacrylate polymer, a methacrylate polymer, or a combination thereof.
5. The coated support of claim 3 wherein the polymer adhesive comprises a two-part epoxy polymer.
6. The coated support of claim 2 wherein the coating composition is derived from reaction of a sol, the sol comprising (i) an alkoxy silane or an organotrialkoxy silane or metasilicate, (ii) a poly(silicone), (iii) a hydrophilic additive, and (iv) the carbonic anhydrase.
7. The coated support of claim 6 wherein the coating composition is derived from reaction of the sol and a catalyst.
8. The coated support of claim 6 wherein the poly(silicone) comprises a poly(siloxane).
9. A process for removing CO 2 from a CO 2 -containing gas, the process comprising
contacting a liquid with a CO 2 -containing gas; and contacting the CO 2 in the liquid with the coated support of claim 2 to catalyze hydration of the CO 2 and form a treated liquid comprising hydrogen ions and bicarbonate ions.
10. The process of claim 9 wherein the hydration of the CO 2 takes place in a reaction vessel comprising a bottom portion containing a gas inlet and a liquid outlet, a top portion containing a liquid inlet and a gas outlet, and a middle portion containing a plurality of the coated supports of claim 2 , the carbonic anhydrase being capable of catalyzing hydration of CO 2 into hydrogen ions and bicarbonate ions.
11. The process of claim 10 wherein the coated supports are packed in the middle portion of the reaction vessel.
12. The coated support of claim 6 wherein the alkoxy silane comprises tetramethylorthosilicate, tetraethylorthosilicate, methyltriethylorthosilicate, ethyltrimethylorthosilicate, dimethyldiethylorthosilicate, tetraglyceryl silicate, or a combination thereof
13. The coated support of claim 6 wherein the organotrialkoxy silane comprises trimethoxymethylsilane, trimethoxyethylsilane, or a combination thereof
14. The coated support of claim 13 wherein the alkoxy silane comprises tetramethylorthosilicate.
15. The coated support of claim 13 wherein the poly(silicone) comprises a poly(siloxane) selected from the group consisting of poly(dimethylsiloxane), poly(dimethylsiloxane)-co-poly(alkene oxide), or a combination thereof.
16. The coated support of claim 14 wherein the poly(siloxane) comprises polydimethylsiloxane.
17. The coated support of claim 15 wherein the poly(silicone) is silanol-terminated.
18. The coated support of claim 2 wherein the hydrophilic additive comprises poly(vinyl alcohol), poly(ethylene oxide), a quaternary ammonium polymer, a crown ether, a cyclodextrin, a surfactant, hexadecyltrimethylammonium bromide, poly(1-methyl-4-vinylpyridinium bromide), poly(acrylamide-methacryloxyethyltrimethylammonium bromide), or a combination thereof.
19. The coated support of claim 6 wherein the hydrophilic additive is a crown ether and the crown ether comprises 12-crown-4, 1,7-diaza-12-crown-4, 1,4,8,11-tetrathiacyclotetradecane, 1,4,8,12-tetraazacyclopentadecane, 15-crown-5, 18-crown-6, dibenzo-18-crown-6, dicyclohexano-18-crown-6, (18-crown-6)-2,3,11,12-tetracarboxylic acid, 1-aza-18-crown-6, diaza-18-crown-6, or a combination thereof.
20. The coated support of claim 19 wherein the crown ether comprises 18-crown-6.
21. The coated support of claim 20 wherein the alkoxy silane comprises tetramethylorthosilicate and the poly(silicone) comprises polydimethylsiloxane.
22. The process of claim 11 wherein the coating composition is derived from reaction of a sol, the sol comprising (i) an alkoxy silane or an organotrialkoxy silane or metasilicate, (ii) a poly(silicone), (iii) a hydrophilic additive, and (iv) the carbonic anhydrase; the alkoxy silane comprises tetramethylorthosilicate, the poly(siloxane) comprises polydimethylsiloxane, and the hydrophilic additive comprises a crown ether.
23. The coated support of claim 6 wherein the alkoxy silane comprises tetramethylorthosilicate, the poly(siloxane) comprises polydimethylsiloxane, and the hydrophilic additive comprises a crown ether.
24. The coated support of claim 6 wherein the coating composition is derived from reaction of a sol, the sol comprising (i) an alkoxy silane or an organotrialkoxy silane or metasilicate, (ii) a poly(silicone), (iii) a hydrophilic additive, and (iv) the carbonic anhydrase; the alkoxy silane comprises tetramethylorthosilicate, the poly(siloxane) comprises polydimethylsiloxane, and the hydrophilic additive comprises a crown ether.
25. The coated support of claim 2 wherein the coating composition has an overall pore volume of at least about 3 μL/g to 500 μL/g.