Burdock fruit extract containing arctigenin and method for producing same
PROBLEM TO BE SOLVED The present invention is intended to provide a burdock fruit extract comprising arctigenin and arctiin at a definite ratio and a method for producing the same. More particularly, the present invention is intended to provide a method for producing a burdock fruit extract comprising arctigenin and arctiin at a weight ratio of approximately 1:1. SOLUTION A method for producing a burdock fruit extract comprising arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 is provided, including the steps of: cutting a burdock fruit and converting arctiin which is inherent in the burdock fruit into arctigenin by enzymatic conversion by beta-glucosidase which is inherent in the burdock fruit, wherein the enzymatic conversion includes reaction at a temperature from 20° C. to 50° C. Also provided is the method further including a step of extracting an extract comprising arctigenin and arctiin by adding an organic solvent and heating to reflux.
1. A method for producing a burdock fruit extract containing arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 from a burdock fruit which inherently comprises arctiin and beta-glucosidase, the method comprising the steps of:
cutting a burdock fruit,
adding water to said cut burdock fruit, and
stirring said cut burdock fruit to a temperature of 20° C. to 50° C. to permit enzymatic conversion of arctiin into arctigenin by beta-glucosidase, to produce a burdock fruit extract, wherein the enzyme activity of said beta-glucosidase is 0.4 U or greater per 1 g of said burdock fruit, and further comprising an extraction step which comprises adding an organic solvent to said burdock fruit extract, wherein the burdock fruit is retained at a temperature of 80° C. or higher during said extraction step to yield the burdock fruit extract containing arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 from a burdock fruit which inherently comprises arctiin and beta-glucosidase.
2. The method according to claim 1 , wherein said burdock fruit is cut to a particle size of from 0.85 mm to 9.5 mm in said cutting step.
3. The method according to claim 1 , wherein said organic solvent is ethanol.