Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
Disclosed are major allergenic proteins in cashew nut, which are legumin-like proteins and 2S albumins. Also disclosed is a polypeptide allergen in the 7S superfamily, which includes vicilin-like and sucrose binding proteins. Several linear epitopes of the cashew nut are identified and characterized. The invention further discloses the sequence of cDNA encoding the allergenic polypeptide, the allergen being designated Ana o 1, and also describes the characterization of the expressed recombinant polypeptide and associated methods employing the polypeptide.
1. A test for detecting a cashew allergy in a patient, said test comprising contacting the skin of a patient with a composition comprising at least one purified polypeptide sequence selected from the group consisting of SEQ ID NOs: 3-14, and 15 and detecting whether the patient exhibits an allergic response indicative of a cashew allergy to the at least one polypeptide sequence.
2. The test of claim 1 , wherein contacting comprises injecting the patient with the purified polypeptide.