Methods and products for tissue repair
Methods and devices for the repair of articular tissue using collagen material are provided. Compositions of collagen material and related kits are also provided.
1. A method comprising: contacting the ends of a ruptured articular tissue in a subject in need thereof with a sterile solution of solubilized collagen and white blood cells, and allowing the solution to set to treat the ruptured articular tissue, wherein the sterile solution comprises solubilized collagen in a concentration of greater than 5 mg/ml and less than or equal to 50 mg/ml.
2. The method of claim 1 , wherein the white blood cells comprise neutrophils.
3. The method of claim 1 , wherein the white blood cells comprise basophils.
4. The method of claim 1 , wherein the white blood cells comprise eosinophils.
5. The method of claim 1 , wherein the white blood cells comprise monocytes.
6. The method of claim 1 , wherein the white blood cells comprise a combination of neutrophils, basophils, eosinophils and monocytes.
7. The method of claim 1 , wherein the sterile solution of solubilized collagen and white blood cells are premixed prior to application to the ruptured articular tissue.
8. The method of claim 1 , wherein the sterile solution of solubilized collagen has a viscosity of 1,000-200,000 centipoise, a hydroxyproline in a concentration of 0.1-5.0 μg/ml, and a neutralizing agent, wherein the solution has an osmolarity of 280-350 mOsm/kg.
9. The method of claim 1 , wherein the solution is a liquid or gel.
10. The method of claim 1 , wherein the solubilized collagen is present in a concentration of greater than 15 mg/ml and less than or equal to 40 mg/ml.
11. The method of claim 1 , wherein the white blood cells are in a concentration of at least 4×10 3 wbc/ml.
12. The method of claim 1 , wherein the sterile collagen solution is lyophilized prior to adding the white blood cells.
13. The method of claim 11 , wherein the sterile collagen solution is lyophilized prior to adding the white blood cells.