Feed supplement with lipase and phytase
View Patent ↗The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5.
1. A method of making a feed supplement comprising mixing a lipolytic enzyme and a phytase, the feed supplement used to increase an uptake of at least one nutrient in a feedstuff to which the feed supplement is added, wherein said lipolytic enzyme has a lipase activity at a pH in a range from pH 1.5 to pH 3.5.
2. The method of claim 1 , further comprising the additional step of homogenising the feed supplement.
3. The method according to claim 1 , further comprising the additional step of pelleting the feed supplement.
4. The method of claim 1 , wherein said lipolytic enzyme comprises one or more of: a polypeptide having sequence of SEQ ID NO: 7 or SEQ ID NO: 8, or a polypeptide having at least 70% identity to SEQ ID NO: 7 or SEQ ID NO: 8; a polypeptide produced by expression of a nucleotide sequence comprising a sequence of SEQ ID NO: 9 or SEQ ID NO: 10; and a sequence having at least 70% identity to SEQ ID NO: 9 or SEQ ID NO: 10.
5. The method of claim 4 , wherein the lipolytic enzyme is produced by expressing a nucleotide sequence comprising a sequece of SEQ ID NO: 9 or SEQ ID NO: 10, or a sequence having at least 70% identity to SEQ ID NO: 9 or SEQ ID NO: 10 in a Trichoderma reesei host cell.
6. The method of claim 1 , wherein the phytase comprises one or more of an E. coli phytase having a sequence of SEQ ID NO: 13 and a Buttiauxella phytase having a sequence of one of SEQ ID NOs 1-6, or a polypeptide having at least 70% identity to one of SEQ ID NOs 1-6, or a polypeptide produced by expression of a nucleotide sequence comprising a sequence of SEQ ID NO: 11, or a sequence comprising nucleotides 253 to 1483 of SEQ ID NO:14, or a sequence that has at least 70% identity to SEQ ID NO: 11 or nucleotides 253 to 1483 of SEQ ID NO:14.
7. The method of claim 1 , wherein said phytase has phytase activity at a pH in a range from pH 2.5 to pH 5.5.
8. A method of making a feedstuff comprising adding to a feed material a feed supplement that comprises at least one phytase and at least one lipase having a lipase activity at a pH in a range from pH 1.5 to pH 3.5, the feedstuff for increasing an availability of at least one nutrient, increasing an available metabolic energy from the feed material, or increasing the availability of the at least one nutrient and increasing the available metabolic energy from the feed material.
9. The method of claim 8 , further comprising adding at least one physiologically acceptable carrier selected from at least one of maltodextrin, limestone (calcium carbonate), cyclodextrin, wheat or a wheat component, sucrose, starch, anti-foam, Na 2 SO 4 , Talc, PVA and mixtures thereof.
10. The method of claim 8 , further comprising homogenising the feed supplement.
11. The method of claim 8 , further comprising pelleting the feed supplement.
12. The method of claim 8 , wherein the feedstuff further comprises at least one low fibre feed material, wherein the at least one low fibre feed material is one of corn, wheat, an animal by-product meal, or soybean.
13. The method of claim 8 , wherein the at least one nutrient is selected from one or more of phosphorous, calcium, amino acids, fat, and starch.