Purified Amphiphilic Peptide Compositions and Uses Thereof
A plurality of amphiphilic peptide chains having alternating hydrophilic and hydrophobic amino acids, wherein the peptide contains at least 8 amino acids, are complementary and structurally compatible, and self-assemble into a beta-sheet macroscopic scaffold wherein peptide at least about 75% of the chains have the same sequence.
1 . A method of promoting wound healing, comprising:
administering to a wound site in a subject a composition comprising a plurality of polypeptides, which plurality constitutes a pure preparation in that:
(i) each of the polypeptides in the plurality is at least 8 amino acids long; and
(ii) each of the polypeptides in the preparation has an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13), or a fragment thereof, wherein the administering is performed such that polypeptides in the plurality assemble into a hydrogel comprising beta-sheets.
2 . The method of claim 1 , further comprising adding an electrolyte to the composition, wherein the polypeptides assemble after addition of the electrolyte.
3 . The method of claim 2 , wherein the electrolyte is added to the composition after administration to the subject.
4 . The method of claim 2 , wherein the electrolyte is added to the composition prior to administration to the subject.
5 . The method of claim 2 , wherein the electrolyte is selected from the group consisting of the following Li, Na, K and Cs.
6 . The method of claim 2 , wherein the electrolyte is added in the form of a solution with a concentration in the range of about 0.1 mM to about 50 mM.
7 . The method of claim 3 , wherein the concentration is at least about 5 mM.
8 . The method of claim 3 , wherein the concentration is at least about 10 mM.
9 . The method of claim 3 , wherein the concentration is at least about 20 mM.
10 . The method of claim 3 , wherein the concentration is at least about 50 mM.
11 . The method of claim 1 , wherein the composition is an aqueous composition.
12 . The method of claim 11 , wherein the concentration of the polypeptides in the aqueous composition is at least about 1% by weight.
13 . The method of claim 11 , wherein the concentration of the polypeptides in the aqueous composition is at least about 2% by weight.
14 . The method of claim 11 , wherein the concentration of the polypeptides in the aqueous composition is at least about 3% by weight.
15 . The method of claim 11 , wherein the concentration of the polypeptides in the aqueous composition is at least about 4% by weight.
16 . The method of claim 11 , wherein the concentration of the polypeptides in the aqueous composition is at least about 5% by weight.
17 . The method of claim 11 , wherein the concentration of the polypeptides in the aqueous composition is at least about 6% by weight.
18 . The method of claim 11 , wherein the concentration of the polypeptides in the aqueous composition is at least about 7% by weight.
19 . The method of claim 11 , wherein the concentration of the polypeptides in the aqueous composition is at least about 8% by weight.
20 . The method of claim 1 , wherein at least about 75% of the polypeptides have an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13).
21 . The method of claim 1 , wherein at least about 80% of the polypeptides have an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13).
22 . The method of claim 1 , wherein at least about 85% of the polypeptides have an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13).
23 . The method of claim 1 , wherein at least about 90% of the polypeptides have an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13).
24 . The method of claim 1 , wherein at least about 95% of the polypeptides have an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13).
25 . The method of claim 1 , wherein at least about 99% of the polypeptides have an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13).
26 . The method of claim 1 , wherein the composition further comprises a buffer.
27 . The method of claim 1 , wherein the composition further comprises a biologically active agent.
28 . The method of claim 1 , wherein the step of administering comprises administering a composition of peptides that are not in a hydrogel state, and allowing the peptides to gel.
29 . The method of claim 1 , wherein the step of administering comprises administering a composition of peptides that are in a gelled state.
30 . The method of either of claim 3 or 28 , wherein the electrolyte is provided by ions present in the subject migrating to the site of administration.
31 . The method of claim 1 , wherein the polypeptides are RADARADARADARADA (SEQ ID NO: 13).
32 . The method of claim 20 , wherein the polypeptides are RADARADARADARADA (SEQ ID NO: 13).
33 . The method of claim 21 , wherein the polypeptides are RADARADARADARADA (SEQ ID NO: 13).
34 . The method of claim 22 , wherein the polypeptides are RADARADARADARADA (SEQ ID NO: 13).
35 . The method of claim 23 , wherein the polypeptides are RADARADARADARADA (SEQ ID NO: 13).
36 . The method of claim 24 , wherein the polypeptides are RADARADARADARADA (SEQ ID NO: 13).
37 . The method of claim 25 , wherein the polypeptides are RADARADARADARADA (SEQ ID NO: 13).
38 . A method of promoting wound healing, comprising:
administering to a wound site in a subject a cell-free composition comprising a plurality of polypeptides, which plurality constitutes a pure preparation in that:
(i) each of the polypeptides in the plurality is at least 8 amino acids long; and
(ii) each of the polypeptides in the preparation has an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13), or a fragment thereof, wherein the administering is performed such that polypeptides in the plurality assemble into a hydrogel comprising beta-sheets.
39 . A method of promoting wound healing, comprising:
administering to a wound site in a subject a composition consisting of a buffer and_a plurality of polypeptides, which plurality constitutes a pure preparation in that:
(i) each of the polypeptides in the plurality is at least 8 amino acids long; and
(ii) each of the polypeptides in the preparation has an amino acid sequence comprising RADARADARADARADA (SEQ ID NO: 13), or a fragment thereof, wherein the administering is performed such that polypeptides in the plurality assemble into a hydrogel comprising beta-sheets.