Detection of microorganisms involved in metabolism of organic environmental pollutants
A method and point of use kit to determine under field conditions the presence and/or amount of a selected Pseudomonas species capable of bioremediating organic environmental pollutants, such as petroleum hydrocarbons.
1. A growth medium selective for growth of only Pseudomonas putida, Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas stutzeri , and/or Pseudomonas fluorescens , the growth medium comprising a peptic digest of animal tissue 10 g/L-30 g/L; magnesium chloride 1 g/L-2 g/L; potassium sulfate 8 g/L-12 g/L; erythromycin 0.005 g/L-0.02 g/L; ampicillin sodium salt 0.005 g/L-0.02 g/L; triclosan 0.02 g/L-0.04 g/L; tetrazolium blue chloride 0.2 g/L-0.5 g/; agar 12 g/L-15 g/L; and excluding glycerol wherein the erythromycin, ampicillin sodium salt, and triclosan are the only antibiotics or antibacterials in the growth medium.
2. A growth medium selective for growth of only Pseudomonas putida, Pseudomonas aeruginosa , and Pseudomonas cepacia , the growth medium comprising a peptic digest of animal tissue 10 g/L-30 g/L; magnesium chloride 1 g/L-2 g/L; potassium sulfate 8 g/L-12 g/L; erythromycin 0.005 g/L-0.02 g/L; ampicillin sodium salt 0.005 g/L-0.02 g/L; triclosan 0.02 g/L-0.04 g/L; tetrazolium blue chloride 0.2 g/L-0.5 g/; agar 12 g/L-15 g/L; and glycerol wherein the erythromycin, ampicillin sodium salt, and triclosan are the only antibiotics or antibacterials in the growth medium.
3. A point-of-use kit to determine the presence or amount of a Psuedomonas species in a potentially contaminated water and/or soil sample, the point-of-use kit comprising
a container containing the growth medium of claim 1 selective for growth limited to at least one Pseudomonas species selected from the group consisting of Pseudomonas putida, Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas stutzeri, Pseudomonas fluorescens , and combinations thereof, and a dye or stain, and
instructions for assessing at least one of the Psuedomonas species in the potentially contaminated water and/or soil sample indicating potential for bioremediation of the potentially contaminated water and/or soil sample.
4. The point-of-use kit of claim 3 where the nutrient medium is a solid medium.
5. The point-of-use kit of claim 3 where the nutrient medium is a liquid medium.
6. The point-of-use kit of claim 3 further comprising an implement to manipulate the potentially contaminated water and/or soil sample.
7. A point-of-use kit to determine the presence or amount of a Psuedomonas species in a potentially contaminated water and/or soil sample, the point-of-use kit comprising a container containing the growth medium of claim 2 selective for growth limited to at least one Pseudomonas species selected from the group consisting of Pseudomonas putida, Pseudomonas aeruginosa, Pseudomonas cepacia , and combinations thereof, and a dye or stain, and instructions for assessing at least one of the Psuedomonas species in the potentially contaminated water and/or soil sample indicating potential for bioremediation of the potentially contaminated water and/or soil sample.
8. The point-of-use kit of claim 7 where the nutrient medium is a solid medium.
9. The point-of-use kit of claim 7 where the nutrient medium is a liquid medium.
10. The point-of-use kit of claim 7 further comprising an implement to manipulate the potentially contaminated water and/or soil sample.