Polypeptides having cellobiohydrolase activitiy and polynucleotides encoding same
The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polynucleotide is operably linked to one or more foreign control sequences that direct production of the polypeptide in an expression host, and wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
2. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
3. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises the sequence of amino acids 19-469 of SEQ ID NO: 2.
4. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 96% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
5. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 98% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
6. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 99% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
7. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by at most ten amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
8. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by at most five amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
9. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by at most three amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
10. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by at most two amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
11. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by one amino acid from the sequence of amino acids 19-469 of SEQ ID NO: 2.
12. A recombinant expression vector comprising the nucleic acid construct of claim 1 .
13. A recombinant host cell comprising the nucleic acid construct of claim 1 .
14. A recombinant host cell transformed with a nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
15. The recombinant host cell of claim 14 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
16. The recombinant host cell of claim 14 , wherein the polypeptide having cellobiohydrolase activity has at least 98% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
17. The recombinant host cell of claim 14 , wherein the polypeptide having cellobiohydrolase activity has at least 99% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
18. The recombinant host cell of claim 17 , wherein the polypeptide having cellobiohydrolase activity comprises the sequence of amino acids 19-469 of SEQ ID NO: 2.