Polypeptides having endoglucanase activity and polynucleotides encoding same
The present invention provides isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cell comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having endoglucanase activity and one or more heterologous control sequences that direct production of the polypeptide in an expression host, wherein the polynucleotide is operably linked to the one or more heterologous control sequences that direct production of the polypeptide in an expression host, and wherein the polypeptide comprises the amino acid sequence of amino acids 24-498 of SEQ ID NO: 2.
2. A method for degrading or converting a cellulosic material, said method comprising:
(i) treating the cellulosic material with an enzyme composition, wherein the enzyme composition comprises a polypeptide having endoglucanase activity; and
(ii) recovering the degraded or converted cellulosic material;
wherein the polypeptide having endoglucanase activity comprises the amino acid sequence of amino acids 24-498 of SEQ ID NO: 2.
3. A method for producing a fermentation product, said method comprising:
(a) saccharifying a cellulosic material with an enzyme composition, wherein the enzyme composition comprises a polypeptide having endoglucanase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation;
wherein the polypeptide having endoglucanase activity comprises the amino acid sequence of amino acids 24-498 of SEQ ID NO: 2.
4. A recombinant host cell comprising the nucleic acid construct of claim 1 .
5. A method of producing a polypeptide having endoglucanase activity, comprising:
(a) cultivating the recombinant host cell of claim 4 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
6. An expression vector comprising the nucleic acid construct of claim 1 .
7. The method of claim 2 , wherein the enzyme composition further comprises an endoglucanase, a GH61 polypeptide having cellulolytic enhancing activity, and a beta-glucosidase.
8. The method of claim 2 , wherein the cellulosic material is pretreated.
9. The method of claim 7 , wherein the cellulosic material is pretreated.
10. The method of claim 3 , wherein the enzyme composition further comprises an endoglucanase, a GH61 polypeptide having cellulolytic enhancing activity, and a beta-glucosidase.
11. The method of claim 3 , wherein the cellulosic material is pretreated.
12. The method of claim 10 , wherein the cellulosic material is pretreated.
13. The method of claim 3 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
14. The method of claim 12 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
15. The method of claim 3 , wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.
16. The method of claim 3 , wherein the fermentation product is ethanol.
17. The method of claim 12 , wherein the fermentation product is ethanol.
18. A recombinant host cell transformed with a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polypeptide comprises the amino acid sequence of amino acids 24-498 of SEQ ID NO: 2, and wherein the polynucleotide is heterologous to the recombinant host cell.
19. A method of producing a polypeptide having endoglucanase activity, comprising:
(a) cultivating the recombinant host cell of claim 18 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.