Genetic testing method and testing apparatus
View Patent ↗A genetic testing method and an apparatus therefor are provided, in which temperatures of a plurality of reaction tubes are independently controlled using a thermostat, a temperature detecting device, and a heating and cooling device provided on each of the reaction tubes, the reaction tubes each accommodate an amplification liquid and a component necessary for amplification, temperature is controlled at individual positions to hold the reaction tubes and at a predetermined temperature set at the individual positions according to an analysis and testing protocol predetermined at individual positions of the reaction tubes, a controlled temperature value is monitored in a reaction tube unit and a corrected value of controlled temperature is computed and stored on a reaction tube unit, a temperature of the reaction tube is controlled based on the computed value, and light emission of the amplification liquid accommodated in the reaction tube is measured.
1. A genetic testing method comprising:
hermetically sealing and stirring a plurality of reaction tubes, each accommodating an amplification liquid including a target nucleic acid to be amplified and a component necessary for amplification;
independently controlling temperatures of each of the reaction tubes using a plurality of thermostats, a plurality of temperature detecting devices, and a plurality of heating and cooling devices independently provided for the reaction tubes to hold each of the reaction tubes at a predetermined temperature individually set for each of the respective reaction tubes according to an analysis and testing protocol predetermined for each of the respective reaction tubes;
monitoring and storing the independently controlled temperatures of each of the reaction tubes;
computing and storing corrected values of the independently controlled temperatures for each of the reaction tubes;
measuring and storing light emission of the amplification liquid accommodated in each of the reaction tubes while independently controlling the temperatures of each of the reaction tubes based on the respective corrected values;
when the corrected values of the independently controlled temperature of one of the reaction tubes exceeds a predetermined range during measurement of light emission, stopping the independent temperature control of the one of the reaction tubes while the remaining reaction tubes respectively continue the independent controlling of the temperatures and the measuring of light emission; and
simultaneously displaying the monitored values of the controlled temperatures and the measured light emission in time series on a display screen in relation to each other including the monitored value of the controlled temperature and the measurement of light emission of the amplification liquid accommodated in the one of the reaction tubes as a display of an abnormal temperature control state.
2. The genetic testing method according to claim 1 ,
wherein an amplification reaction is performed by a PCR method.
3. The genetic testing method according to claim 1 ,
wherein an amplification reaction is performed by a LAMP method.
4. The genetic testing method according to claim 1 , further comprising:
when a difference between one of the corrected values at a first time and another of the corrected values at a second, earlier time for one of the reaction tubes exceeds a predetermined range, outputting an alarm.
5. The genetic testing method according to claim 1 , further comprising:
after finishing the measurement of light emission of the amplification liquid accommodated in another one of the reaction tubes, disposing of the other one of the reaction tubes in a disposal box.