HIGHLY IMMUNOGENIC HIV P24 SEQUENCES
The invention relates to peptides comprising part or all of a conserved element within a Center-of-Tree (COT) sequence derived from a family of polypeptides encoded by naturally occurring variants of HIV. The invention also relates to immunogenic compositions and vaccines comprising said peptides. The invention also relates to methods for the identification of HIV controller patients based on the detection of the T cells of the patient to mount a cytotoxic T cell response against said peptides and to methods for the identification of immunogenic peptides within a family of variant polypeptides.
1 - 25 . (canceled)
26 . A peptide having the capacity to induce a cytotoxic T lymphocyte (CTL) response when administered to a subject, the peptide having a sequence that comprises part or all of a conserved element within a Center-of-Tree (COT) sequence derived from a family of polypeptides encoded by naturally occurring variants of HIV.
27 . A peptide according to claim 26 , having a length of 10 amino acids or less.
28 . A peptide according to claim 27 , wherein the naturally occurring variants of HIV belong to group M HIV.
29 . A peptide according to claim 28 , wherein the family of polypeptides encoded by naturally occurring variants of HIV is formed by polypeptides encoded by the gag gene.
30 . A peptide according to claim 29 , wherein the polypeptides encoded by the gag gene are p24 polypeptides.
31 . A peptide according to claim 30 , wherein the conserved element has a sequence selected from the group consisting of SEQ ID NO:1 to 14.
32 . A peptide according to claim 31 , wherein the peptide has a sequence selected from the group consisting of SEQ ID NO:15 to 36.
33 . A polynucleotide encoding a peptide according to claim 26 .
34 . A vector comprising a polynucleotide according to claim 33 .
35 . An immunogenic composition or a vaccine comprising a peptide according to claim 26 , wherein the composition or vaccine is capable of generating a cytotoxic T lymphocyte response when administered to a subject.
36 . An immunogenic composition or a vaccine comprising at least one of the following items (i) to (iii):
(i) a peptide of sequence SEQ ID NO:26,
(ii) at least one peptide of the group consisting of the peptides of sequence SEQ ID NO:27 to 32, and
(iii) at least one peptide of the group consisting of the peptides of sequence SEQ ID NO:33 to 36,
wherein the composition or vaccine is capable of generating a cytotoxic T lymphocyte response when administered to a subject.
37 . An immunogenic composition or a vaccine comprising a polynucleotide according to claim 33 , wherein the composition or vaccine is capable of generating a cytotoxic T lymphocyte response when administered to a subject.
38 . An immunogenic composition or a vaccine comprising a vector according to claim 34 , wherein the composition or vaccine is capable of generating a cytotoxic T lymphocyte response when administered to a subject.
39 . A method for the treatment or prevention of a disease resulting from HIV-infection in a subject, the method comprising administration to said subject of a peptide according to claim 26 .
40 . A method for the treatment or prevention of a disease resulting from HIV-infection in a subject, the method comprising administration to said subject of a polynucleotide according to claim 33 .
41 . A method for the treatment or prevention of a disease resulting from HIV-infection in a subject, the method comprising administration to said subject of a vector according to claim 34 .
42 . A method for the treatment or prevention of a disease resulting from HIV-infection in a subject, the method comprising administration to said subject of an immunogenic composition or a vaccine according to claim 35 .
43 . A method for the treatment or prevention of a disease resulting from HIV-infection in a subject, the method comprising administration to said subject of an immunogenic composition or a vaccine according to claim 36 .
44 . An antibody or a polypeptide comprising an antigen-binding region thereof that binds specifically to a peptide according to claim 26 .
45 . A method for identification of a HIV controller patient, the method comprising testing capability of a sample containing T-cells isolated from said subject to mount a cytotoxic T lymphocyte (CTL) response against at least one 10mer peptide derived from a conserved element within a Center-of-Tree (COT) sequence of an HIV protein, and identifying a patient capable of mounting a CTL response against said at least one 10mer peptide that is higher than a response against at least one 10mer peptide in a reference sample as a HIV controller patient.
46 . A method for identification of immunogenic peptides within a family of variant polypeptides, the method comprising the steps of
(i) obtaining the center-of-tree sequence of the family of variant polypeptides,
(ii) identifying conserved sequence elements within the center-of-tree sequence,
(iii) producing peptides the sequence of which is comprised within said conserved sequence elements, and
(iv) testing the peptides obtained in step (iii) for their immunogenicity.