Antibodies to tumor endothelial marker 8
The present invention is directed to particular antibodies and fragments thereof that find use in the detection, prevention and treatment of diseases and disorders associated with abnormal angiogenesis. In particular, these antibodies detect tumor endothelial marker 8 (TEM8) in its native and cell-surface expressed form. Also disclosed are improved methods for producing monoclonal antibodies, as well as pharmaceutical compositions and kits.
1. A humanized or chimeric antibody or antibody fragment comprising a heavy chain and light chain; wherein the heavy chain comprises the amino acid sequences of SEQ ID NO:45 (CDR1), SEQ ID NO:46 (CDR2), and SEQ ID NO:47 (CDR3), and the light chain comprises the amino acid sequences of SEQ ID NO:48 (CDR4), SEQ ID NO:49 (CDR5), and SEQ ID NO:50 (CDR6); wherein the antibody or antibody fragment binds immunologically to native cell-surface expressed Tumor Endothelial Marker 8 (TEM8).
2. The humanized or chimeric antibody of claim 1 , wherein said antibody is a human IgG antibody.
3. The humanized or chimeric antibody or antibody fragment of claim 1 , wherein said light chain amino acid sequence comprises SEQ ID NO:36.
4. The humanized or chimeric antibody or antibody fragment of claim 1 , wherein said heavy chain amino acid sequence comprises SEQ ID NO:43.
5. The humanized or chimeric antibody or antibody fragment of claim 1 , comprising a light chain amino acid sequence comprising SEQ ID NO:36 and a heavy chain amino acid sequence comprising SEQ ID NO:43.
6. The humanized or chimeric antibody of claim 1 , wherein said antibody or antibody fragment is conjugated or fused to a drug, wherein the drug is a chemotherapeutic, a radiotherapeutic, a thrombogenic agent, an immunomodulatory domain, a lymphocyte binding domain, or a toxin.