Autoluminescent phytosensor plants and uses thereof
Real-time monitoring of plant or environmental conditions is solved by Autoluminescent Phytosensor Plants (ALPS) disclosed herein that emit light in response to a specified stimulus or condition, which light emission is detected or measured by a sensor.
1. A transplastomic plant cell containing a LUX operon comprising LUX genes integrated within a plastidal genome therein,
wherein any or all of said LUX genes are separated by an intercistronic expression element (IEE) operably linked thereto,
wherein expression of said LUX genes is enhanced by a heterologous translational leader sequence operably linked to one or more of said LUX genes,
wherein said heterologous translational leader sequence is a T7g10 leader sequence, and
wherein said transplastomic plant cell is selected from the group consisting of a species of petunia and a species of Nicotiana.
2. A method of producing an autoluminescent plant comprising: introducing into a plant cell plastid an expression cassette containing a LUX operon comprising LUX genes,
wherein any or all of said LUX genes are separated by an intercistronic expression element (IEE) operably linked thereto,
wherein expression of said LUX genes is enhanced by a heterologous translational leader sequence operably linked to one or more of said LUX genes,
wherein said heterologous translational leader sequence is a T7g10 leader sequence,
wherein said plant cell plastid is regenerated into a mature transplastomic plant, and
wherein said transplastomic plant is selected from the group consisting of a species of petunia and a species of Nicotiana.
3. A transplastomic plant of claim 2 , wherein the transplastomic plant is chosen from Petunia cv. “Perfectunia Blue”, Nicotiana Alata cv. “Whisper Rose Shades”, or Nicotiana Sylvestris cv. “Only the Lonely”.