Decellularization and recellularization of solid organs
The invention provides for methods and materials to decellularize a solid organ and to recellularize such a decellularized organ to thereby generate a solid organ.
1. A perfusion decellularized extracellular matrix portion of a pig, bovine, sheep, or canine organ comprising a non-vasculature decellularized extracellular matrix and vasculature decellularized extracellular matrix, wherein said vasculature decellularized extracellular matrix retains a majority of fluid introduced to said vasculature decellularized extracellular matrix, wherein said vascular decellularized extracellular matrix comprises human cells, wherein said organ is a kidney, lung, liver, pancreas, or spleen, and wherein prior to decellularization the perfusion decellularized extracellular matrix portion included about 10,000,000 cells/mg tissue or wherein the perfusion decellularized extracellular matrix portion is capable of being reseeded with at least 10,000,000 cells.
2. A perfusion decellularized solid organ of a pig, bovine, sheep, or canine comprising a non-vasculature decellularized extracellular matrix and vasculature decellularized extracellular matrix, wherein said vasculature decellularized extracellular matrix retains a majority of fluid introduced to said vasculature decellularized extracellular matrix, wherein said vascular decellularized extracellular matrix comprises human cells, wherein said perfusion decellularized solid organ is a kidney, lung, liver, pancreas, or spleen, and wherein prior to decellularization a perfusion decellularized portion included about 10,000,000 cells/mg tissue or wherein the perfusion decellularized solid organ is capable of being reseeded with at least 10,000,000 cells.
3. The perfusion decellularized extracellular matrix portion of claim 1 , wherein said human cells are regenerative cells.
4. The perfusion decellularized extracellular matrix portion of claim 1 , wherein said human cells are autologous to a patient.
5. The perfusion decellularized extracellular matrix portion of claim 1 , wherein said human cells are allogenic to a patient.
6. The perfusion decellularized extracellular matrix portion of claim 1 , wherein said human cells are progenitor cells.
7. The perfusion decellularized extracellular matrix portion of claim 1 , wherein said human cells are stem cells.
8. The perfusion decellularized solid organ of claim 2 , wherein said human cells are regenerative cells.
9. The perfusion decellularized solid organ of claim 2 , wherein said human cells are autologous to a patient.
10. The perfusion decellularized solid organ of claim 2 , wherein said human cells are allogenic to a patient.
11. The perfusion decellularized solid organ of claim 2 , wherein said human cells are progenitor cells.
12. The perfusion decellularized solid organ of claim 2 , wherein said human cells are stem cells.
13. A perfusion decellularized solid organ or portion thereof of a pig, bovine, sheep, or canine comprising a cannula, non-vasculature decellularized extracellular matrix, and vasculature decellularized extracellular matrix, wherein said vasculature decellularized extracellular matrix retains a majority of fluid introduced to said vasculature decellularized extracellular matrix, wherein said vascular decellularized extracellular matrix comprises cells, wherein said perfusion decellularized solid organ or portion thereof is a kidney, lung, liver, pancreas, or spleen, and wherein prior to decellularization a perfusion decellularized portion included about 10,000,000 cells/mg tissue or wherein the perfusion decellularized solid organ or portion thereof is capable of being reseeded with at least 10,000,000 cells.
14. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein said cells are regenerative cells.
15. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein said cells are autologous to a patient.
16. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein said cells are allogenic to a patient.
17. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein said cells are progenitor cells.
18. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein said cells are stem cells.
19. The perfusion decellularized extracellular matrix portion of claim 1 , wherein the pre-perfusion decellularized extracellular matrix portion prior to decellularization included about 10,000,000 cells/mg tissue.
20. The perfusion decellularized extracellular matrix portion of claim 1 , wherein the decellularized extracellular matrix portion is capable of being reseeded with at least 10,000,000 cells.
21. The perfusion decellularized solid organ of claim 2 , wherein the portion of the pre-perfusion decellularized solid organ prior to decellularization included about 10,000,000 cells/mg tissue.
22. The perfusion decellularized solid organ of claim 2 , wherein the perfusion decellularized solid organ is capable of being reseeded with at least 10,000,000 cells.
23. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein the portion of a pre-perfusion decellularized solid organ included about 10,000,000 cells/mg tissue.
24. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein the perfusion decellularized solid organ or portion is capable of being reseeded with at least 10,000,000 cells.
25. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein the perfusion decellularized solid organ or portion is a solid organ.
26. The perfusion decellularized solid organ or portion thereof of claim 13 , wherein the perfusion decellularized solid organ or portion is a portion of the solid organ.