Virus like particle purification
Methods for purifying human Calciviruses are disclosed, including Noroviruses and Sapoviruses.
1. A method of purifying Calicivirus virus-like particles (VLPs) comprising contacting a solution containing said VLPs with at least one chromatographic material, wherein the at least one chromatographic material is an ion exchange chromatographic material, wherein the chromatographic materials used in the method do not comprise hydroxyapatite, and wherein the VLPs are purified to greater than about 70%.
2. The method of claim 1 , wherein the ion exchange chromatographic material is an anion exchange chromatographic material.
3. The method of claim 2 , wherein the anion exchange chromatographic material is a DEAE-Sepharose FF resin.
4. The method of claim 2 , wherein the method further comprises the use of a cation exchange chromatographic material.
5. The method of claim 1 , wherein the method further comprises the use of a hydrophobic interaction chromatographic material.
6. The method of claim 1 , wherein the method further comprises a precipitation step.
7. The method of claim 6 , wherein the precipitation step is a PEG precipitation step.
8. The method of claim 7 , wherein the PEG precipitation step is carried out prior to contacting the solution containing the VLPs with the ion exchange chromatographic material.
9. The method of claim 1 , wherein the VLPs are purified to greater than about 90%.
10. The method of claim 1 , wherein the method further comprises an ultrafiltration and/or diafiltration step.
11. The method of claim 1 , wherein the Calicivirus VLPs are Norovirus VLPs.
12. The method of claim 11 , wherein the Norovirus VLPs are Norovirus genogroup I or genogroup II VLPs.
13. The method of claim 12 , wherein the Norovirus genogroup I VLPs are Norovirus genotype I.1 VLPs.