Methods and compositions for inducing deregulation of EPHA7 and ERK phosphorylation in human acute leukemias
View Patent ↗Methods for assessing a pathological condition in a subject include measuring one or more markers where a difference is indicative of acute lymphoblastic leukemia (ALL) or a predisposition to ALL, uses and compositions are disclosed.
1. A method to interfere with an acute lymphoblastic leukemia (ALL) response signaling pathway in at least one leukemic cell producing an ALL1/AF9 chimeric fusion protein, wherein the method comprises:
administering, to at least one K562 leukemic cell, 5-iodotubericidin and an ALL1/AF9-specific siRNA consisting of “SEMj” which suppresses expression of an ALL1/AF9 chimeric fusion protein, thereby interfering with an acute lymphoblastic leukemia (ALL) response signaling pathway.
2. The method of claim 1 , wherein the at least one leukemic cell is in a living organism.
3. The method of claim 2 , wherein the organism is a human.
4. A method to interfere with an acute lymphoblastic leukemia (ALL) response signaling pathway in at least one leukemic cell producing an ALL1/AF9 chimeric fusion protein,
comprising administering to at least one leukemic cell, 5-iodotubercidin and an ALL1/AF9-specific siRNA which suppresses expression of an ALL1/AF9 chimeric fusion protein, thereby interfering with an acute lymphoblastic leukemia (ALL) response signaling pathway.
5. The method of claim 4 , wherein the ALL1/AF9-specific siRNA is a SEMj siRNA.