Formulation and Method for Preparing Specific T Cell, and Method for Preparing the Formulation
The present invention provides a formulation and method for preparing specific T cells, and a method for fabricating the above formulation is also disclosed. The formulation can induce specific T cell responses and comprises at least a cell population of dendritic killer cells presenting specific antigens. In addition, the method mentioned above for preparing specific T cells comprises following steps. A cell population of T cells is provided at first. And then, a formulation of preparing specific T cells is added to mix with the cell population of T cells. After cultivating, the specific T cells are harvested. Furthermore, the method for fabricating the above formulation comprises the following steps. First, a cell population of dendritic killer cells is provided. A target sample is then provided, and a step of making the cell population of the dendritic killer cells co-cultivate with the target sample is performed. After co-cultivating, a cell population of dendritic killer cells presenting specific antigens is harvested.
1 . A formulation for preparing specific T cells comprising at least a cell population of dendritic killer cells presenting specific antigens.
2 . The formulation according to claim 1 further comprising a physiologically acceptable medium.
3 . The formulation according to claim 1 further comprising a physiologically acceptable buffer.
4 . The formulation according to claim 1 , wherein a concentration of the cell population of dendritic killer cells presenting specific antigens is 10 6 cells/mL.
5 . The formulation according to claim 1 , wherein the specific antigens are tumor-specific antigens.
6 . A method for preparing a formulation of preparing specific T cells comprising the following steps:
providing a cell population of dendritic killer cells;
providing a target sample;
making the cell population of dendritic killer cells co-cultivate with the target sample; and
harvesting a cell population of dendritic killer cells presenting specific antigens after co-cultivating.
7 . The method according to claim 6 , wherein the cell population of the dendritic killer cells processes cytotoxicity against the target sample and presents a specific antigens of the target sample on its surface to form the cell population of dendritic killer cells presenting specific antigens.
8 . The method according to claim 6 , wherein the target sample is tumor cells from a cancer patient.
9 . The method according to claim 8 , wherein the specific antigens are tumor-specific antigens.
10 . The method according to claim 8 , wherein the target sample and the cell population of dendritic killer cells are both gathered from the cancer patient.
11 . The method according to claim 6 , wherein the cell population of dendritic killer cells is generated ex vivo by culturing dendritic killer cells from a human blood sample with a cytokine, and the cytokine comprises IL-15.
12 . The method according to claim 6 , wherein the method is a platform for screening specific antigens.
13 . A method for preparing specific T cells comprising the following steps:
providing a cell population of T cells;
adding a formulation of preparing specific T cells and mix with the cell population of T cells; and
harvesting the specific T cells after cultivating.
14 . The method according to claim 13 , wherein the formulation comprises a cell population of dendritic killer cells presenting specific antigens.
15 . The method according to claim 14 , wherein the cell population of T cells is activated by the cell population of dendritic killer cells presenting specific antigens to form the specific T cells.
16 . The method according to claim 13 , wherein the specific antigens are tumor-specific antigens.
17 . The method according to claim 16 , wherein the specific T cells are tumor-specific T cells.
18 . A composition containing a plurality of tumor antigen-pulsed human DKCs, wherein the plurality of tumor antigen-pulsed human DKCs is obtained by contacting a population of human DKCs with cells derived from a tumor, the population of human DKCs being HLA-G − CD14 − CD19 − CD3 − CD56 + HLA-DR + .
19 . A method of generating a plurality of tumor antigen-specific CD8 + T cells, the method comprising contacting a population of CD8 + T cells with the composition of claim 18 , thereby generating a plurality of tumor antigen-specific CD8 + T cells.
20 . A method of treating a tumor in a human subject in need thereof, the method comprising:
providing a plurality of tumor antigen-pulsed human DKCs that is prepared by contacting a population of human DKCs with cells derived from a tumor in the subject, the population of DKCs being HLA-G − CD14 − CD19 − CD3 − CD56 + HLA-DR + ;
contacting a population of CD8 + T-cells with the plurality of tumor antigen-pulsed human DKCs to generate tumor antigen-specific CD8 + T cells; and
delivering the tumor antigen-specific CD8 + T cells to the human subject.