Methods for improved treatment of adipose tissue
The present disclosure relates generally to methods of treatment of tissue prior to implantation. In one aspect, the methods of treatment include washing adipose tissue with detergents to improve the viability of adipose cells for implantation and/or to increase the amount of viable adipose cells per volume of tissue for implantation.
1. A method for treating tissue comprising:
selecting a tissue comprising adipocytes for implantation;
contacting the tissue with a detergent at a concentration and time sufficient to remove non-viable materials comprising blood, water, and particulate tissue matrix from the tissue to produce an adipose tissue comprising adipocytes and adipose extracellular tissue matrix, wherein a poloxamer is not used in the contacting step; and
rinsing the tissue to remove the detergent, such that the contacting and rinsing increase the concentration of viable adipocytes in the tissue as compared to the tissue prior to treatment.
2. The method according to claim 1 , wherein the method is performed prior to implantation of the tissue during autologous fat transfer.
3. The method according to claim 1 , wherein the detergent is a nonionic detergent.
4. The method according to claim 1 , wherein the detergent is a biocompatible detergent.
5. The method according claim 1 , wherein the detergent comprises a polysorbate.
6. The method according to claim 1 , wherein the detergent comprises a polyethylene glycol.
7. The method according to claim 1 , wherein the non-viable materials further comprise pharmaceutical agents and epinephrine.
8. The method according to claim 1 , wherein the tissue is contacted with the detergent and rinsed under conditions selected to reduce the water content of the tissue.
9. The method according to claim 1 , further comprising agitating the tissue while the tissue is in contact with the detergent.
10. The method of claim 9 , wherein agitating the tissue comprises at least one of stirring, shaking, and causing the detergent to flow in contact with the tissue.
11. The method according to claim 1 , further comprising repeating the steps of contacting the tissue with the detergent and rinsing the tissue.
12. The method according to claim 1 , wherein the tissue is rinsed with a rinsing fluid comprising a saline solution.
13. The method according to claim 1 , further comprising implanting the tissue in a patient.
14. The method according to claim 1 , further comprising removing water from the tissue.
15. The method according to claim 1 , wherein the contacting and rinsing at least doubles the concentration of viable adipocytes in the tissue as compared to the tissue prior to treatment.