IP Library Granted Patent US 9,422,544
Granted Patent B2
US 9,422,544 · App. 13/931,110 · Granted Aug 23, 2016

Methods and kits for extraction of DNA

Inventors: Maxim Brevnov (Union City, CA); Hemant Pawar (Ann Arbor, MI); Manohar Furtado (San Ramon, CA); Jaiprakash Shewale (Santa Clara, CA)
Assignee: LIFE TECHNOLOGIES CORPORATION
C12N15/1013C12N15/1006C12Q1/6806
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Quick Facts
Patent No.
US 9,422,544
App. No.
13/931,110
Granted
Aug 23, 2016
Kind
B2
Abstract

Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

Claims (7)

1. A composition comprising:

a non-covalent complex comprising a nucleic acid, a polymer comprising dextran, and magnetically attractable particles comprising dextran encased magnetite nanoparticles, and

a detergent, comprising N-lauroyl sarcosine or lauroyl sarcosinate.

2. The composition of claim 1 , wherein the dextran is in the range of 1-5 mg/ml.

3. The composition of claim 1 , wherein the dextran has a molecular weight of 5,000,000 to 40,000,000.

4. The composition of claim 1 , wherein the lauroyl sarconsinate is in the range of about 5-15%.

5. The composition of claim 1 , wherein the dextran encased particles are in the range of about 2-10 mg/ml.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 22, 2016
From: BREVNOV, MAXIM; PAWAR, HEMANT; FURTADO, MANOHAR; SHEWALE, JAIPRAKASH
To: LIFE TECHNOLOGIES CORPORATION
Reel/Frame 039499/0306 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 24, 2013
From: BREVNOV, MAXIM; PAWAR, HEMANT; FURTADO, MANOHAR; SHEWALE, JAIPRAKASH
To: APPLIED BIOSYSTEMS, LLC
Reel/Frame 031474/0215 →
Continuity (3)
Continuation 12371515 · Feb 13, 2009
Provisional Application 61028986 · Feb 15, 2008
Related Publication 20140039176A1 · Feb 6, 2014