Plants with increased activity of a starch phosphorylating enzyme
View Patent ↗The present invention relates to plant cells and plants, which are genetically modified, whereby the genetic modification leads to an increase in the activity of a starch-phosphorylating OK1 protein in comparison to the corresponding wild type plant cells or wild type plants that have not been genetically modified. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesize a modified starch. Therefore, the present invention also concerns the starches synthesized from the plant cells and plants according to the invention, methods for manufacturing these starches, and the manufacture of starch derivatives of these modified starches, as well as flours containing starches according to the invention. Furthermore, the present invention also relates to nucleic acids, coding starch-phosphorylating OK1 proteins, vectors, host cells, plant cells, and plants containing such nucleic acid molecules. In addition, the present invention relates to OK1 proteins that have starch-phosphorylating activity.
1. A modified starch obtained from a genetically modified plant comprising one or more genetically modified plant cells that exhibit increased activity in at least one OK1 protein in comparison to corresponding wild type plant cells that have not been genetically modified, wherein said OK1 protein is:
(a) encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, or the complementary sequence thereof;
(b) encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 1;
(c) the amino acid sequence of SEQ ID NO: 2;
(d) an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2;
(e) encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 2;
(f) encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 3, or the complementary sequence thereof;
(g) encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 3;
(h) the amino acid sequence of SEQ ID NO: 4;
(i) an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 4; or
(j) encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 4, and
wherein the modified starch has an increased proportion of starch phosphate bonded in the C-3 position compared with starch phosphate bonded in the C-6 position in comparison to starch obtained from wild-type plants that have not been genetically modified.
2. The modified starch of claim 1 , wherein said OK1 protein is encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, or the complementary sequence thereof.
3. The modified starch of claim 1 , wherein said OK1 protein is encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 1.
4. The modified starch of claim 1 , wherein said OK1 protein comprises the amino acid sequence of SEQ ID NO: 2.
5. The modified starch of claim 1 , wherein said OK1 protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2.
6. The modified starch of claim 1 , wherein said OK1 protein is encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 2.
7. The modified starch of claim 1 , wherein said OK1 protein is encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 3, or the complementary sequence thereof.
8. The modified starch of claim 1 , wherein said OK1 protein is encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 3.
9. The modified starch of claim 1 , wherein said OK1 protein comprises the amino acid sequence of SEQ ID NO: 4.
10. The modified starch of claim 1 , wherein said OK1 protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 4.
11. The modified starch of claim 1 , wherein said OK1 protein is encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 4.
12. A method of manufacturing a modified starch according to claim 1 , comprising extracting the starch from a genetically modified plant comprising one or more genetically modified plant cells that exhibit increased activity in at least one OK1 protein in comparison to corresponding wild type plant cells that have not been genetically modified.
13. A method of manufacturing a modified starch according to claim 1 , comprising extracting the starch from a harvestable plant part of a genetically modified plant comprising one or more genetically modified plant cells that exhibit increased activity in at least one OK1 protein in comparison to corresponding wild type plant cells that have not been genetically modified.
14. A method of manufacturing a derived starch comprising deriving a modified starch according to claim 1 .
15. A method of manufacturing a modified starch comprising extracting the starch from a genetically modified plant cell that exhibits increased activity in at least one OK1 protein in comparison to corresponding wild type plant cells that have not been genetically modified, wherein said OK1 protein is:
(a) encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, or the complementary sequence thereof;
(b) encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 1;
(c) the amino acid sequence of SEQ ID NO: 2;
(d) an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2;
(e) encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 2;
(f) encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 3, or the complementary sequence thereof;
(g) encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 3;
(h) the amino acid sequence of SEQ ID NO: 4;
(i) an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 4; or
(j) encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 4.
16. The method of claim 15 , wherein said OK1 protein is encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, or the complementary sequence thereof.
17. The method of claim 15 , wherein said OK1 protein is encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 1.
18. The method of claim 15 , wherein said OK1 protein comprises the amino acid sequence of SEQ ID NO: 2.
19. The method of claim 15 , wherein said OK1 protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2.
20. The method of claim 15 , wherein said OK1 protein is encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 2.
21. The method of claim 15 , wherein said OK1 protein is encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 3, or the complementary sequence thereof.
22. The method of claim 15 , wherein said OK1 protein is encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 3.
23. The method of claim 15 , wherein said OK1 protein comprises the amino acid sequence of SEQ ID NO: 4.
24. The method of claim 15 , wherein said OK1 protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 4.
25. The method of claim 15 , wherein said OK1 protein is encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 4.
26. A method for manufacturing a flour comprising grinding plant cells that exhibit increased activity in at least one OK1 protein in comparison to corresponding wild type plant cells that have not been genetically modified, wherein said OK1 protein is:
(a) encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, or the complementary sequence thereof;
(b) encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 1;
(c) the amino acid sequence of SEQ ID NO: 2;
(d) an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2;
(e) encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 2:
(f) encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 3, or the complementary sequence thereof;
(g) encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 3;
(h) the amino acid sequence of SEQ ID NO: 4;
(i) an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 4; or
(j) encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 4.
27. A flour comprising the starch of claim 1 .
28. A flour comprising the starch of claim 2 .
29. A flour comprising the starch of claim 3 .
30. A flour comprising the starch of claim 4 .
31. A flour comprising the starch of claim 5 .
32. A flour comprising the starch of claim 6 .
33. A flour comprising the starch of claim 7 .
34. A flour comprising the starch of claim 8 .
35. A flour comprising the starch of claim 9 .
36. A flour comprising the starch of claim 10 .
37. A flour comprising the starch of claim 11 .
38. A method for manufacturing a flour comprising grinding plant parts that exhibit increased activity in at least one OK1 protein in comparison to corresponding wild type plant cells that have not been genetically modified, wherein said OK1 protein is:
(a) encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, or the complementary sequence thereof;
(b) encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 1;
(c) the amino acid sequence of SEQ ID NO: 2;
(d) an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2;
(e) encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 2;
(f) encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 3, or the complementary sequence thereof;
(g) encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 3;
(h) the amino acid sequence of SEQ ID NO: 4;
(i) an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 4; or
(j) encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 4.
39. The method of claim 38 , wherein said OK1 protein is encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1, or the complementary sequence thereof.
40. The method of claim 38 , wherein said OK1 protein is encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 1.
41. The method of claim 38 , wherein said OK1 protein comprises the amino acid sequence of SEQ ID NO: 2.
42. The method of claim 38 , wherein said OK1 protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2.
43. The method of claim 38 , wherein said OK1 protein is encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 2.
44. The method of claim 38 , wherein said OK1 protein is encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 3, or the complementary sequence thereof.
45. The method of claim 38 , wherein said OK1 protein is encoded by a nucleic acid molecule having at least 95% identity to the nucleotide sequence of SEQ ID NO: 3.
46. The method of claim 38 , wherein said OK1 protein comprises the amino acid sequence of SEQ ID NO: 4.
47. The method of claim 38 , wherein said OK1 protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 4.
48. The method of claim 38 , wherein said OK1 protein is encoded by a nucleic acid molecule coding a protein having the amino acid sequence of SEQ ID NO: 4.