Diagnosis, prognosis, and treatment of myocardial infarction
Methods are provided for diagnosis, prognosis, and treatment associated with a cardiovascular event in a subject as well as methods of providing anti-platelet therapy. The methods can comprise obtaining a sample from the subject, and determining the presence of at least two single nucleotide polymorphisms (SNP) in the sample selected from Rs7541936 in PTGER3, Rs977214 in PTGER3, and Rs2206343 in PTGER3. The methods can also comprise determining the presence in the sample of Rs4383756 in PTGER4. Some methods comprise administering conventional anti-platelet therapy to the subject if Rs4383756 in PTGER4 is present in the sample. Furthermore, some methods comprise administering an EP3 antagonist and/or conventional anti-platelet therapy to the subject if Rs4383756 in PTGER4 is not present in the sample. The cardiovascular event can involve thrombosis or an embolic event, a myocardial infarction, a stroke, a primary cardiovascular event, or coronary artery disease.
1. A kit for diagnosis, prognosis, and treatment associated with a cardiovascular event and/or anti-platelet therapy in a subject, comprising:
a set of two, three, or four single nucleotide polymorphism (SNP)-specific probes wherein each SNP-specific probe is selected from the group consisting of:
a probe for detecting Rs7541936 in PTGER3 in a sample obtained from the subject;
a probe for detecting Rs977214 in PTGER3 in a sample obtained from the subject;
a probe for detecting Rs2206343 in PTGER3 in a sample obtained from the subject; and
a probe for detecting Rs4383756 in PTGER4 in a sample obtained from the subject;
the kit including the probe for detecting Rs4383756 in PTGER4 in the set of probes and/or an EP3 receptor antagonist;
wherein the kit does not include any probes in addition to the set of probes; and
wherein the probes are fluorescently-labeled.
2. The kit of claim 1 , comprising: the SNP-specific probe for detecting Rs4383756 in PTGER4 in a sample obtained from the subject.
3. The kit of claim 1 , comprising a set of SNP-specific probes consisting of three or four probes selected from the group consisting of:
a probe for detecting Rs7541936 in PTGER3 in a sample obtained from the subject;
a probe for detecting Rs977214 in PTGER3 in a sample obtained from the subject,
a probe for detecting Rs2206343 in PTGER3 in a sample obtained from the subject; and
a probe for detecting Rs4383756 in PTGER4 in a sample obtained from the subject.
4. The kit of claim 1 , comprising a set of SNP-specific probes consisting of:
a probe for detecting Rs7541936 in PTGER3 in a sample obtained from the subject;
a probe for detecting Rs977214 in PTGER3 in a sample obtained from the subject,
a probe for detecting Rs2206343 in PTGER3 in a sample obtained from the subject; and
a probe for detecting Rs4383756 in PTGER4 in a sample obtained from the subject.
5. The kit of claim 1 , comprising an EP3 receptor antagonist.
6. The kit of claim 5 , wherein the EP3 receptor antagonist is DG-041.
7. The kit of claim 1 , comprising a set of SNP-specific probes consisting of:
a probe for detecting Rs7541936 in PTGER3 in a sample obtained from the subject;
a probe for detecting Rs977214 in PTGER3 in a sample obtained from the subject; and
a probe for detecting Rs2206343 in PTGER3 in a sample obtained from the subject.
8. The kit of claim 1 , and further comprising an apparatus capable of identifying a SNP in the sample.
9. The kit of claim 1 , wherein each probe is a single nucleic acid molecule.