Method for determining presence of carcinomic cells with 5, 10, 15, 20-tetrakis (carboxyphenyl) porphine
Presented is a method of determining the presence of carcinomic cells in a sample by contacting a sample of cells with a solution of TCPP to permit binding of the TCPP to components of the abnormal dysplastic or carcinomic cells, if any are present; detecting TCPP fluorescence in the sample, the presence of TCPP fluorescence being indicative that the sample contains dysplastic or carcinomic cells.
1. A method to determine if a sample of cells contains dysplastic or carcinomic cells, the method comprising the steps of:
contacting the sample with a solution of 5,10,15,20-tetrakis(carboxyphenyl) porphine (TCPP) under conditions permitting binding of the TCPP to components of the dysplastic or carcinomic cells, if any are present, wherein the solution of TCPP comprises the TCPP pre-dissolved in basified alcohol; and
detecting TCPP fluorescence in the sample, the presence of TCPP fluorescence being indicative that the sample contains dysplastic or carcinomic cells.