Methods for producing biological materials
Methods of producing biological materials from cells and organisms are provided. Aspects of the methods include modulating the stress conditions of the cells and/or organism to produce biological materials having one or more desired properties. In certain aspects, the cell or organism is evaluated to detect the presence or absence of a stressed phenotype, wherein an unstressed phenotype may be produced before the cell or organism produces the biological material of interest. The biological materials produced from such cells and organisms may be used for a variety of applications, including therapeutic, research, and other applications.
1. A method for producing a biological product from a cell, the method comprising:
culturing the cell containing a nucleic acid encoding at least a portion of the biological product, under conditions that modulate a stressed phenotype of the cell and allow for expression of the biological product, wherein the produced biological product is different from the biological product produced in a control cell having the stressed phenotype as compared to the cell;
evaluating the cell to identify the absence of the stressed phenotype; and
recovering the biological product.
2. The method according to claim 1 , wherein evaluating the cell to identify the absence of a stressed phenotype is performed prior to recovering the biological product.
3. The method according claim 1 , comprising producing an unstressed phenotype in the cell.
4. The method according to claim 3 , wherein producing an unstressed phenotype comprises changing the cell's nutritional intake by changing the quantity or type of food available to the cell.
5. The method according to claim 3 , wherein producing an unstressed phenotype comprises changing the cell's environment.
6. The method according to claim 5 , wherein the unstressed phenotype is produced by changing the cell's temperature or the cell's exposure to light.
7. The method according to claim 3 , comprising administering to the cell an agent to reduce stress.
8. The method according to claim 3 , comprising evaluating the cell to identify the absence of a stressed phenotype, wherein the evaluating comprises visually inspecting the cell.
9. The method according to claim 3 , comprising evaluating the cell to identify the absence of a stressed phenotype, wherein the evaluating comprises genomic assessment or proteomic assessment of the cell.
10. The method according to claim 1 , wherein the cell is mammalian.
11. The method according to claim 1 , wherein the cell is obtained from a multicellular organism having an unstressed phenotype.
12. The method according to claim 11 , wherein the unstressed phenotype is produced in the organism by changing the organism's nutritional intake.
13. The method according to claim 11 , wherein the unstressed phenotype is produced in the organism by changing the organism's environment.
14. The method according to claim 13 , wherein the unstressed phenotype is produced in the organism by changing the organism's temperature or the organism's exposure to light.
15. The method according to claim 11 , comprising evaluating the organism to identify the unstressed phenotype.
16. The method according to claim 1 , wherein the biological product is an antibody.
17. The method according to claim 1 , wherein the biological product is a peptide.
18. The method according to claim 1 , wherein evaluating the cell to identify the absence of the stressed phenotype is performed prior to producing the biological product.
19. The method according to claim 1 , wherein the produced biological product has improved safety as compared to the biological product produced in the control cell having a stressed phenotype as compared to the cell.
20. The method according to claim 1 , wherein the produced biological product has improved efficacy as compared to the biological product produced in the control cell having a stressed phenotype as compared to the cell.