Analysis device and analysis method using the same
View Patent ↗Provided are an analysis device and an analysis method. According to the device and the method, a giant magnetoresistance (GMR) sensor unit is formed to be the same as the size of one cancer cell or smaller and magnetic resistance according to the number of magnetic nano particles coupled with the one cancer cell by using the GMR sensor unit, thereby not only diagnosing cancer but also simply and cheaply distinguishing the type of the cancer.
1. An analysis device comprising:
a giant magnetoresistance (GMR) sensor unit disposed on a substrate;
a receptor-fastened layer fastening receptors to the GMR sensor unit and disposed on the GMR sensor unit;
the receptors fastened to the receptor-fastened layer;
a target particle comprising a plurality of magnetic nano particles and a plurality of markers, at least one of the plurality of magnetic nano particles being coupled with a corresponding one of the receptors using a first antibody and being coupled with a corresponding one of the markers of the target particle using a second antibody; and
electrodes in contact with both ends of the GMR sensor unit,
wherein a width of the GMR sensor unit is the same as or smaller than a width of the target particle, and
wherein the target particle is a single particle.
2. The device of claim 1 , wherein the receptor-fastened layer is formed of polymer.
3. The device of claim 1 , wherein a magnetic resistance, a signal voltage of the GMR sensor unit, or both, changes depending on the number of the magnetic nano particles coupled with the target particle captured by the receptors.
4. The device of claim 3 , wherein the target particle is a cancer cell, and
wherein the magnetic nano particles are attached to epithelial cellular adhesion molecules (EpCAMs) expressed in a cellular membrane of the cancer cell.
5. The device of claim 4 , wherein the cancer cell comprises a different number of EpCAMs according to a type thereof, and
wherein the number of the magnetic nano particles coupled with the cancer cell corresponds to the number of EpCAMs.
6. The device of claim 1 , wherein sensitivity S of the GMR sensor unit and a distance R from a surface of the GMR sensor unit satisfy the following equation:
S∝ 1/ R 3 .
7. The device of claim 1 , further comprising a selection device connected to one end of the GMR sensor unit,
wherein a unit analysis cell includes the GMR sensor unit and the selection device, and
wherein the analysis device comprises a plurality of unit analysis cells.
8. The device of claim 7 , wherein the unit analysis cells are arranged in an array.
9. The device of claim 1 , further comprising a channel through which a mixed solution comprising the target particle flows,
wherein the GMR sensor unit and the electrodes are arranged in the channel.
10. The device of claim 9 , wherein the mixed solution is blood, and
wherein the target particle is a blood cancer cell.
11. The device of claim 7 , wherein the selection device is one of a transistor and a diode.
12. The device of claim 1 , wherein the receptors are fastened to only a portion of the receptor-fastened layer, the portion overlapping with the GMR sensor unit.